Human CORO1C (Coronin-1C) knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
CORO1C KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.
View Alternative Names
COR1C_HUMAN, CORO 1C, CRNN 4, Coronin actin binding protein 1C, Coronin-1C, Coronin-3, HCRNN 4
- Sanger seq
Unknown
Sanger Sequencing - Human CORO1C (Coronin-1C) knockout HEK-293T cell lysate (AB258377)
Allele-1 : Insertion of the selection cassette in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human CORO1C (Coronin-1C) knockout HEK-293T cell lysate (AB258377)
Allele-2 : 2 bp insertion in exon 2
- WB
Lab
Western blot - Human CORO1C (Coronin-1C) knockout HEK-293T cell lysate (AB258377)
Blocking buffer and concentration : ½ volume of Odyssey Blocking Buffer (TBS)+ ½ volume of 0.1% TBS Diluting buffer and concentration : ½ volume of Odyssey Blocking Buffer (TBS)+ ½ volume of 0.1% TBST False colour image of Western blot : Anti-Coronin-1C antibody [EPR25365-24] (ab283693) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab283693 was shown to bind specifically to Coronin-1C. A band was observed at 53 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in Coronin-1C knockout cell line ab266381 (knockout cell lysate (ab258377). To generate this image, wild-type and Coronin-1C knockout HEK-293T cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-Coronin-1C antibody [EPR25365-24] (<a href='/en-us/products/primary-antibodies/coronin-1c-antibody-epr25365-24-ab283693'>ab283693</a>) at 1/1000 dilution
Lane 1:
Wild-type 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2:
Western blot - Human CORO1C (Coronin-1C) knockout HEK-293T cell lysate (ab258377) at 20 µg
Lane 2:
Western blot - Human CORO1C (Coronin-1C) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-coro1c-coronin-1c-knockout-hek-293t-cell-line-ab266381'>ab266381</a>)
Lane 2:
Western blot - Human CORO1C (Coronin-1C) knockout HEK-293T cell lysate (ab258377)
Secondary
Lanes 1 - 2:
Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution
Lanes 1 - 2:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB283693
Anti-Coronin-1C antibody [EPR25365-24]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size
![Western blot - Anti-Coronin-1C antibody [EPR25365-24] (AB283693)](https://content.abcam.com/products/images/coronin-1c-antibody-epr25365-24-ab283693--western-blot-img401874.jpg)
- 0
- 0
Cell Lines & Lysates
AB266381
Human CORO1C (Coronin-1C) knockout HEK-293T cell line
Advanced Validation
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com