Human COX7A2 knockout HEK-293T cell lysate
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COX7A2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.
View Alternative Names
COX7AL, COX7AL1, COXVIIa L, CX7A2_HUMAN, Cytochrome c oxidase polypeptide 7A2 mitochondrial, Cytochrome c oxidase polypeptide VIIa liver/heart, Cytochrome c oxidase subunit 7A2, Cytochrome c oxidase subunit 7A2 mitochondrial, Cytochrome c oxidase subunit VIIa polypeptide 2, Cytochrome c oxidase subunit VIIa-L, Cytochrome c oxidase subunit VIIa-liver/heart, Hepatic cytochrome c oxidase chain VIIa, MGC118950, MGC118951, MGC118952, MGC126875, MGC126877, VIIAL, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human COX7A2 knockout HEK-293T cell lysate (AB258826)
Allele-2 : Insertion of the selection cassette in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human COX7A2 knockout HEK-293T cell lysate (AB258826)
Allele-1 : 1 bp insertion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
COX7A2 contributes to the cytochrome c oxidase (complex IV) of the mitochondrial electron transport chain. This complex is important for ATP production through oxidative phosphorylation. COX7A2 forms part of the multi-subunit complex that facilitates efficient transfer of electrons which is essential for mitochondrial energy production. Its function in this complex underlines its importance in maintaining cellular respiration and energy homeostasis.
Pathways
COX7A2 plays an important role in the mitochondrial oxidative phosphorylation pathway. This pathway is essential for ATP production in eukaryotic cells. Another significant pathway involving COX7A2 is the respiratory electron transport pathway. Within these pathways COX7A2 interacts with other cytochrome c oxidase subunits like COX4 and COX5A which help regulate electron transfer and influence overall metabolic activity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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