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AB258828

Human CPA4 knockout HeLa cell lysate

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CPA4 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 6.
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Sanger Sequencing - Human CPA4 knockout HeLa cell lysate (AB258828)
  • Sanger seq

Unknown

Sanger Sequencing - Human CPA4 knockout HeLa cell lysate (AB258828)

Homozygous : Insertion of the selection cassette in exon 6

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 6.

Disease

Adenocarcinoma

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Complementary Products

Cell Lines & Lysates

AB265831

Human CPA4 knockout HeLa cell line

Sanger Sequencing - Human CPA4 knockout HeLa cell line (AB265831)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
CPA4
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPA4 also known as Carboxypeptidase A4 is a type of zinc metalloprotease enzyme. It weighs about 47 kDa. CPA4 is primarily found in tissues like the prostate ovaries and placenta. The protein plays a role in processing the C-terminal ends of various proteins which is essential for the maturation and stabilization of other peptides and proteins. Its substrate specificity is similar to other metallocarboxypeptidases but may exhibit unique functions due to its expression profiles.
Biological function summary

Carboxypeptidase A4 contributes to the breakdown of peptides by removing terminal amino acids. It can operate as a single enzyme or potentially associate with other factors though specific complex formations with CPA4 are not well-documented. This proteolytic activity can regulate peptide hormone activity and aid in the final stages of protein digestion. The activity of CPA4 is also influenced by its interaction with zinc ions which are necessary for its catalytic function.

Pathways

CPA4 takes part in metabolic and digestive enzyme pathways. It specifically relates to pancreatic digestive pathways where it interacts with other proteins such as Carboxypeptidase B and C. These enzymes together coordinate the processing of dietary proteins converting them into amino acids readily absorbable by the body. CPA4's precise role in these pathways helps maintain digestive efficiency and nutrient absorption.

CPA4 has associations with some types of cancer such as prostate cancer possibly related to its expression in prostate tissue. Researchers have also explored links between CPA4 activity and the development of certain genetic disorders. Associations between CPA4 and insulin-like growth factor-binding proteins suggest pathways through which CPA4 might influence cell growth and proliferation potentially connecting it to oncogenic processes.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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