Human CPNE1 knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human CPNE1 knockout HEK-293T cell lysate (AB258829)
Homozygous : 26 bp deletion in exon 2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CPNE1 interacts with membrane lipids and proteins playing a significant role in diverse cellular processes. It is part of a larger complex associated with membrane trafficking and cytoskeletal organization. CPNE1 can influence cellular processes like cell division and motility through its interactions with other proteins often regulating intracellular signaling pathways.
Pathways
CPNE1 is involved in calcium-mediated signaling and the phosphoinositide 3-kinase (PI3K) pathway. The protein interacts with Akt a downstream target of the PI3K pathway therefore influencing cell survival and growth. These interactions underline CPNE1's role in essential cellular functions linking it with other proteins like phospholipase C.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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