Human CPT1A knockout HEK-293T cell lysate
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(0 Publication)
- WB
Lab
Western blot - Human CPT1A knockout HEK-293T cell lysate (AB256880)
Lane 1 : Wild-type HEK-293T cell lysate (20µg)
Lane 2 : CPT1A knockout HEK-293T cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab220789 observed at 88 kDa. Red - loading control ab8245 observed at 37 kDa.
ab220789 Anti-CPT1A antibody [EPR21843-71-1C] was shown to specifically react with CPT1A in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266319 (knockout cell lysate ab256880) was used. Wild-type and CPT1A knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab220789 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-CPT1A antibody [EPR21843-71-1C] (<a href='/en-us/products/primary-antibodies/cpt1a-antibody-epr21843-71-1c-ab220789'>ab220789</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
CPT1A knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human CPT1A knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-cpt1a-knockout-hek-293t-cell-line-ab266319'>ab266319</a>)
Predicted band size: 88 kDa
Observed band size: 88 kDa
false
- WB
Lab
Western blot - Human CPT1A knockout HEK-293T cell lysate (AB256880)
Lane 1 : Wild-type HEK-293T cell lysate (20µg)
Lane 2 : CPT1A knockout HEK-293T cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab234111 observed at 88 kDa. Red - loading control ab8245 observed at 37 kDa.
ab234111 Anti-CPT1A antibody [EPR21843-71-2F] was shown to specifically react with CPT1A in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266319 (knockout cell lysate ab256880) was used. Wild-type and CPT1A knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab234111 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (<a href='/en-us/products/primary-antibodies/cpt1a-antibody-epr21843-71-2f-ab234111'>ab234111</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
CPT1A knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human CPT1A knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-cpt1a-knockout-hek-293t-cell-line-ab266319'>ab266319</a>)
Predicted band size: 88 kDa
Observed band size: 88 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CPT1A knockout HEK-293T cell lysate (AB256880)
Allele-1 : 17 bp deletion in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human CPT1A knockout HEK-293T cell lysate (AB256880)
Allele-2 : Insertion of the selection cassette in exon 3
Complementary Products
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CPT1A plays a vital role in energy production by facilitating mitochondrial fatty acid oxidation. It does not function as a solitary enzyme but often associates with other enzymes forming a multiprotein complex that includes acyl-CoA synthetase. This complex enables efficient fatty acid transport and oxidation converting stored fats into usable energy. As CPT1A primarily operates in the liver its activity significantly impacts overall lipid and energy homeostasis demonstrating its critical regulatory role.
Pathways
CPT1A is central to the fatty acid beta-oxidation pathway an important process for breaking down fatty acids to produce energy. This pathway also involves proteins such as CPT1B a related isoform present in muscle tissues. CPT1A's function is important in the liver's capacity to regulate energy balance and respond to metabolic demands. Additionally CPT1A interacts within the signaling pathway of AMPK (AMP-activated protein kinase) which further integrates it into broader metabolic regulation networks linking energy status to cellular function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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