JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB257180

Human CPT2 knockout HeLa cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

CPT2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.
3 Images
Western blot - Human CPT2 knockout HeLa cell lysate (AB257180)
  • WB

Lab

Western blot - Human CPT2 knockout HeLa cell lysate (AB257180)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : CPT2 knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab181114 observed at 74 kDa. Red - loading control ab8245 observed at 37 kDa.
ab181114 Anti-CPT2/CPT1 antibody [EPR13626] - C-terminal was shown to specifically react with CPT2/CPT1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265931 (knockout cell lysate ab257180) was used. Wild-type and CPT2/CPT1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab181114 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (<a href='/en-us/products/primary-antibodies/cpt2-antibody-epr13626-c-terminal-ab181114'>ab181114</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CPT2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CPT2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cpt2-knockout-hela-cell-line-ab265931'>ab265931</a>)

Predicted band size: 74 kDa

Observed band size: 74 kDa

false

Sanger Sequencing - Human CPT2 knockout HeLa cell lysate (AB257180)
  • Sanger seq

Unknown

Sanger Sequencing - Human CPT2 knockout HeLa cell lysate (AB257180)

Allele-1 : 2 bp deletion in exon1

Sanger Sequencing - Human CPT2 knockout HeLa cell lysate (AB257180)
  • Sanger seq

Unknown

Sanger Sequencing - Human CPT2 knockout HeLa cell lysate (AB257180)

Allele-2 : 1 bp deletion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.

Disease

Adenocarcinoma

style
left-column

Complementary Products

Primary Antibodies

AB181114

Anti-CPT2 antibody [EPR13626] - C-terminal

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (AB181114)

  • 2
  • 1
Cell Lines & Lysates

AB265931

Human CPT2 knockout HeLa cell line

Advanced Validation

Western blot - Human CPT2 knockout HeLa cell line (AB265931)

  • 0
  • 0
Proteins & Peptides

AB114539

Recombinant Human CPT2 protein

SDS-PAGE - Recombinant Human CPT2 protein (AB114539)

  • 0
  • 0
Assay Kits

AB112117

Cell Cycle Assay Kit (Fluorometric - Red)

Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Red) (AB112117)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257180-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human CPT2 knockout HeLa cell lysate", "number":"AB257180-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB257180-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
CPT2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Carnitine palmitoyltransferase 2 (CPT2) also known as CPT-2 is an enzyme located mainly in the mitochondria of liver muscle and heart tissues. This enzyme has a molecular mass of approximately 68 kDa. CPT2 plays a mechanical role in the transfer of long-chain fatty acids from the cytosol into the mitochondrial matrix completing the process initiated by CPT1 on the outer mitochondrial membrane. CPT2 acts on the inner mitochondrial membrane converting acylcarnitine back into acyl-CoA which is an essential step in beta-oxidation. Expression of CPT2 is higher in tissues with elevated fatty acid oxidation rates.
Biological function summary

CPT2 is integral to the mitochondrial pathway of fatty acid metabolism. It functions as part of the carnitine shuttle system a complex important for the conversion of acylcarnitine to acyl-CoA inside the mitochondria. This role is vital for the full oxidation of long-chain fatty acids. Through its action CPT2 aids in maintaining energy homeostasis and producing ketone bodies especially during periods of fasting or strenuous exercise.

Pathways

CPT2 is involved in both the beta-oxidation and the carnitine shuttle pathways. These pathways facilitate the energy production from fatty acids particularly during states of low carbohydrate availability. CPT2 works closely with CPT1 as both enzymes form the gateway for the entry of long-chain fatty acids into mitochondria. Together with enzymes from the tricarboxylic acid cycle CPT2 helps sustain ATP production under such metabolic conditions.

Defects in CPT2 can lead to disorders like CPT2 deficiency which present with symptoms of muscle weakness and recurrent rhabdomyolysis especially during prolonged fasting or significant exercise. This deficiency can also result in hypoketotic hypoglycemia and liver dysfunction. CPT2-related disorders often involve altered interactions with proteins such as the acyl-CoA dehydrogenases impacting the normal fatty acid oxidation process and causing metabolic disturbances.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com