Human CRAT knockout HEK-293T cell lysate
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CRAT KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon6 and 5 bp deletion in exon6.
View Alternative Names
CACP_HUMAN, Carnitine O-acetyltransferase, Carnitine acetylase, Carnitine acetyltransferase, CrAT
- Sanger seq
Unknown
Sanger Sequencing - Human CRAT knockout HEK-293T cell lysate (AB258378)
Allele-1 : 5 bp deletion in exon6
- Sanger seq
Unknown
Sanger Sequencing - Human CRAT knockout HEK-293T cell lysate (AB258378)
Allele-2 : 1 bp deletion in exon6
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Carnitine O-acetyltransferase facilitates the transportation of acyl groups across the mitochondrial membranes influencing energy homeostasis by converting excess acyl-CoA to acylcarnitine. It does not form part of a larger protein complex but operates as a singular catalytic entity. The enzyme's function in balancing acyl-CoA and free CoA levels is essential for maintaining cellular metabolic flexibility allowing cells to switch energy sources efficiently when necessary.
Pathways
Carnitine O-acetyltransferase sits within the fatty acid oxidation pathway aiding in the linkage between carbohydrate and lipid metabolism. It interacts with proteins like carnitine palmitoyltransferase I (CPT I) and acetyl-CoA carboxylase important for controlling the flow of long-chain fatty acids into mitochondria for β-oxidation. The action of CRAT impacts the ketogenesis pathway assisting in maintaining energy production through conversion of stored fats when glucose is not readily available.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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