CTSH KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon3.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon3.
Alternative names
ACC 4, ACC 5, Aleurain, CATH_HUMAN, CPSB, CTSH, Cathepsin B3, Cathepsin BA, Cathepsin H heavy chain, Cathepsin H light chain, Cathepsin H mini chain, Minichain, N-benzoylarginine-beta-naphthylamide hydrolase, pro cathepsin H
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CTSH KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon3.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon3.
- Concentration
Properties
- Gene name
- CTSH
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cathepsin H also known as CPSH is a lysosomal cysteine protease that plays a role in intracellular protein degradation. It has a molecular mass of approximately 28 kilodaltons. This enzyme is expressed across various tissues with high expression levels in liver kidney and spleen. The enzyme primarily functions at acidic pH levels within lysosomes where it acts to break down peptides into amino acids by removing terminal residues.
- Biological function summary
As a member of the cathepsin family Cathepsin H contributes to the regulation of protein turnover and homeostasis. It performs a dual role as both an aminopeptidase and endopeptidase. In addition to its function in protein degradation the enzyme involves itself in antigen processing during immune responses. Reactivity of Cathepsin H in immune complexes shows its importance in shaping immune tolerance and responses.
- Pathways
Cathepsin H interacts with the lysosomal degradation pathway and plays a role in the MHC class II-mediated antigen presentation. This pathway is pivotal for the regulation of immune responses. Relation to other proteins like Cathepsin L and Cathepsin B highlights Cathepsin H's complementary function in proteolytic processing within lysosomes. Its role and expression play an integral part in balancing protein synthesis and degradation cycles.
- Associated diseases and disorders
Cathepsin H associates closely with cancer and neurodegenerative diseases. Its activity can influence tumor progression and metastasis due to its role in extracellular matrix remodeling and cell migration. In neurodegenerative disorders such as Alzheimer's disease its dysregulated activity may contribute to neuronal cell death. Cathepsin H’s connection to proteins like amyloid precursor protein (APP) in Alzheimer's highlights the complex interplay between protease activity and disease mechanisms.
Product promise
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2 product images
Western blot - Human CTSH (Cathepsin H) knockout HEK-293T cell lysate (ab258387)
Lane 1:Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 2:CTSH knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 3:HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate (20 ug)ab88960 was shown to specifically react with Cathepsin H in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line Human CTSH (Cathepsin H) knockout HEK-293T cell line ab266245 (knockout cell lysate ab258387) was used. Wild-type and Cathepsin H knockout samples were subjected to SDS-PAGE. ab88960 and Anti-GAPDH antibody[EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Anti-Cathepsin H antibody [AF14D7] (ab88960) at 1/1000 dilution
Lane 1: Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2: CTSH knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2: Western blot - Human CTSH (Cathepsin H) knockout HEK-293T cell line (Human CTSH (Cathepsin H) knockout HEK-293T cell line ab266245)
Lane 3: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution
Sanger Sequencing - Human CTSH (Cathepsin H) knockout HEK-293T cell lysate (ab258387)
Homozygous: 11 bp deletion in exon3
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