Human CTSL (Cathepsin L/MEP) knockout HEK-293T cell lysate
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CTSL KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.
View Alternative Names
CATL, CATL1_HUMAN, CTSL, CTSL1, Cathepsin L, Cathepsin L1, Cathepsin L1 light chain, FLJ31037, MEP, MGC123162, Major excreted protein, cathepsin L, 1 b, cb15, ctsl1b, hgg1, wu:fb30g09
- WB
Supplier Data
Western blot - Human CTSL (Cathepsin L/MEP) knockout HEK-293T cell lysate (AB257213)
False colour image of Western blot : anti CTSL antibody staining at 1/200 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in red. A band was observed at 25 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in anti CTSL antibody knockout cell line ab266521 (knockout cell lysate ab257213). To generate this image, wild-type and anti CTSL antibody knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
false
- Sanger seq
Unknown
Sanger Sequencing - Human CTSL (Cathepsin L/MEP) knockout HEK-293T cell lysate (AB257213)
Homozygous : 2 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Cathepsin L links to cellular homeostasis and extracellular matrix remodeling. It often acts in protein turnover and antigen processing within endolysosomal compartments making it essential for major histocompatibility complex class II presentation. Cathepsin L forms complexes in certain conditions playing roles in interacting and modifying other proteins. It controls processes essential for cell survival differentiation and apoptosis.
Pathways
Cathepsin L plays significant roles in pathways like apoptosis and autophagy. It coordinates with other proteases and proteins such as cathepsin B and cathepsin S to regulate cell death and survival. In apoptosis cathepsin L mediates the breakdown of cellular components working alongside caspases. Its interaction with autophagy involves degradation of long-lived proteins highlighting its role in recycling amino acids during stress conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB266521
Human CTSL (Cathepsin L/MEP) knockout HEK-293T cell line
Advanced Validation
- 0
- 0
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (AB133641)](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-ab133641--western-blot-img83583.jpg)
Primary Antibodies
AB24170
Anti-LAMP1 antibody - Lysosome Marker
BOND RX™ Validated|Lab Essentials
- 4
- 100
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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