Human CUTC knockout HeLa cell lysate
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CUTC KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.
View Alternative Names
CGI 32, CUTC_HUMAN, Copper homeostasis protein cutC homolog, CutC copper transporter homolog (E. coli), RP11-483F11.3
- WB
Unknown
Western blot - Human CUTC knockout HeLa cell lysate (AB258388)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : CUTC knockout HeLa cell lysate (20 ug)
Lane 3 : Jurkat cell lysate (20 ug)
Lane 4 : SH-SY5Y cell lysate (20 ug)
ab133762 was shown to specifically react with CUTC in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265860 (knockout cell lysate ab258388) was used. Wild-type and CUTC knockout samples were subjected to SDS-PAGE. ab133762 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
false
- Sanger seq
Unknown
Sanger Sequencing - Human CUTC knockout HeLa cell lysate (AB258388)
Allele-2 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human CUTC knockout HeLa cell lysate (AB258388)
Allele-1 : 2 bp deletion in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Copper homeostasis protein CUTC functions in the regulation of intracellular copper levels and may interact with copper chaperones. The protein does not work in isolation but instead might form complexes with other copper-binding proteins to facilitate efficient copper regulation. Proper function of CUTC is necessary for various copper-dependent enzymatic activities highlighting its role in maintaining cellular copper sufficiency.
Pathways
CUTC is engaged in the copper homeostasis pathway that ensures adequate copper distribution and detoxification. This protein is associated with other copper-related proteins like ATP7A/B which transport copper across cellular membranes. Through these interactions CUTC assists in protecting cells from copper toxicity and supports essential biological functions requiring copper.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-CUTC antibody [EPR7929] (AB133762)](https://content.abcam.com/products/images/cutc-antibody-epr7929-ab133762--western-blot-img424118.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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