Human CXCL1 (GRO alpha) knockout HeLa cell lysate
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(0 Publication)
- WB
Lab
Western blot - Human CXCL1 (GRO alpha) knockout HeLa cell lysate (AB257079)
Lane 1 : Wild-type HeLa Treated TNF alpha (5 ng/mL, 6 h) + BFA (5 μg/mL, 6 h) cell lysate 20 μg
Lane 2 : CXCL1 knockout HeLa Treated TNF alpha (5 ng/mL, 6 h) + BFA (5 μg/mL, 6 h) cell lysate 20 μg
False colour image of Western blot : Anti-CXCL1/GRO alpha antibody [EPR19892] staining at 1/100 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab206411 was shown to bind specifically to CXCL1/GRO alpha. A band was observed at 8 kDa in treated wild-type HeLa cell lysates with no signal observed at this size in CXCL1 knockout cell line ab261774 (knockout cell lysate ab257079). To generate this image, wild-type and CXCL1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 20 minutes exposure time. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and HRP conjugated Goat anti-Mouse (H+L) at 1/20000 dilution.
All lanes:
Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (<a href='/en-us/products/primary-antibodies/cxcl1-gro-alpha-antibody-epr19892-ab206411'>ab206411</a>) at 1/100 dilution
All lanes:
Western blot - Human CXCL1 (GRO alpha) knockout HeLa cell lysate (ab257079) at 20 µg
Predicted band size: 11 kDa
Observed band size: 8 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CXCL1 (GRO alpha) knockout HeLa cell lysate (AB257079)
Allele-1 : 62 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human CXCL1 (GRO alpha) knockout HeLa cell lysate (AB257079)
Allele-2 : Insertion of the selection cassette in exon2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.
Pathways
CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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