AB275531

Human CXCR5 knockout Raji cell lysate

Name: Human CXCR5 knockout Raji cell lysate (ab275531) | Abcam
Brand: Abcam Limited
SKU: AB275531
Price: 670 USD
Availability: InStock

Be the first to review this product! Submit a review

(0 Publication)

CXCR5 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2.

View Alternative Names

BLR 1, Burkitt lymphoma receptor 1, Burkitt lymphoma receptor 1 GTP binding protein, Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5), C-X-C chemokine receptor type 5, CD 185, CD185 antigen, CXCR5_HUMAN, Chemokine (C-X-C motif) receptor 5, Chemokine C X C motif receptor 5, Chemokine receptor 5, MDR-15, MGC117347, Monocyte-derived receptor 15

2 Images

Lab

Western blot - Human CXCR5 knockout Raji cell lysate (AB275531)

Lane 1 : Wild-type Raji cell lysate 30 ug
Lane 2 : CXCR5 knockout Raji cell lysate 30 ug
Lane 3 : Daudi cell lysate 30 ug
Lane 4 : Jurkat cell lysate 30 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab254415 observed at 60 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab254415 was shown to react with CXCR5 in wild-type Raji cells in Western blot with loss of signal observed in CXCR5 knockout sample. Wild-type and CXCR5 knockout Raji cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab254415 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 ^°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-CXCR5 antibody [EPR23463-30] (<a href='/en-us/products/primary-antibodies/cxcr5-antibody-epr23463-30-ab254415'>ab254415</a>) at 1/1000 dilution

Lane 1:

Wild-type Raji cell lysate at 30 µg

Lane 2:

CXCR5 knockout Raji cell lysate at 30 µg

Lane 2:

Western blot - Human CXCR5 knockout Raji cell line (<a href='/en-us/products/cell-lines/human-cxcr5-knockout-raji-cell-line-ab273380'>ab273380</a>)

Lane 3:

Daudi cell lysate at 30 µg

Lane 4:

Jurkat cell lysate at 30 µg

Predicted band size: 42 kDa

false

Sanger Sequencing - Human CXCR5 knockout Raji cell lysate (AB275531)

Sanger seq

Supplier Data

Sanger Sequencing - Human CXCR5 knockout Raji cell lysate (AB275531)

Homozygous : 19 bp deletion in exon 2

Key facts

Cell type

Raji

Species or organism

Human

Tissue

Lymphatic

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2

Disease

Lymphoma

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

style

left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab275531-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human CXCR5 knockout Raji cell lysate", "number":"AB275531-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type Raji cell lysate", "number":"AB275531-CMP01", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Gene name

CXCR5

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing, Western blot

Zygosity

Homozygous

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCR5 also known as CD185 is a chemokine receptor with a mass of approximately 43 kDa. It is part of the G protein-coupled receptor family and is widely expressed on follicular B helper T (Tfh) cells B cells and a subset of circulating blood cells. CXCR5 is recognized as a marker for Tfh cells which are critical for the function and maturation of B cells within germinal centers. In tissues CXCR5 expression appears prominently in regions where immune cell interactions facilitate immune and inflammatory responses.

Biological function summary

CXCR5 serves a significant role in directing B cells and Tfh cells to lymphoid tissues by interacting with its ligand CXCL13. This interaction involves recruitment and organization of B cells into follicles within secondary lymphoid organs. The presence of CXCR5 allows B cells and Tfh cells to navigate the follicular zones efficiently enhance antigen-specific immune responses and contribute to long-lived humoral immunity. CXCR5 functions as part of a complex that includes signaling pathways influencing cellular movements and location.

Pathways

CXCR5 plays a significant part in the immune system's trafficking pathways particularly within the crosstalk between chemokines and their receptors. CXCR5 expression facilitates the CXC chemokine pathway which important for positioning immune cells within lymphoid organs. Additionally CXCR5 interacts with proteins such as CXCL13 working together to pattern the architecture of germinal centers where affinity maturation and class switching of antibodies occur critical processes in adaptive immunity.

CXCR5 has been implicated in autoimmune diseases and certain lymphomas. For example aberrant CXCR5 expression is linked to the pathogenesis of diseases like systemic lupus erythematosus (SLE) where it might contribute to immune dysregulation and chronic inflammation. In the context of certain lymphomas CXCR5's role in lymphoid organ architecture and cell migration becomes even more relevant. Its expression often parallels the presence of CXCL13 hinting at a network of interactions promoting tumor survival and growth within lymphoid landscapes. Understanding these relationships can offer insight into developing therapeutic strategies potentially involving CXCR5 inhibitors or anti-CXCR5 antibodies to address these diseases.

style

left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style

left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Suspension

Gender

Male

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CXCR5 knockout Raji cell lysate

Western blot - Human CXCR5 knockout Raji cell lysate (AB275531)

Sanger Sequencing - Human CXCR5 knockout Raji cell lysate (AB275531)

Key facts

Cell type

Species or organism

Tissue

Knockout validation

Mutation description

Disease

Reactivity data

Product details

What's included?

Properties and storage information

Gene name

Gene editing type

Gene editing method

Knockout validation

Zygosity

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Quality control

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

Product protocols

Complementary Products

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Product promise