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AB273777

Human CYP24A1 knockout A549 cell lysate

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CYP24A1 KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 98%.
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Next Generation Sequencing - Human CYP24A1 knockout A549 cell lysate (AB273777)
  • NGS

Supplier Data

Next Generation Sequencing - Human CYP24A1 knockout A549 cell lysate (AB273777)

Knockout achieved by CRISPR/Cas9; X = 1 bp deletion; Frameshift : 98%

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Next Generation Sequencing

Mutation description

Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 98%

Disease

Carcinoma

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Complementary Products

Proteins & Peptides

AB152322

Recombinant Human CYP24A1 protein

SDS-PAGE - Recombinant Human CYP24A1 protein (AB152322)

  • 0
  • 0
Primary Antibodies

AB125001

Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (AB125001)

  • 5
  • 5
Primary Antibodies

AB26

Anti-p53 antibody [PAb 240]

KO Validated|Lab Essentials

Western blot - Anti-p53 antibody [PAb 240] (AB26)

  • 4
  • 56
Cell Lines & Lysates

AB273735

Human CYP24A1 knockout A549 cell line

Next Generation Sequencing - Human CYP24A1 knockout A549 cell line (AB273735)

  • 0
  • 0
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
CYP24A1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP24A1 also known as cytochrome P450 family 24 subfamily A member 1 is an enzyme with a molecular mass of approximately 56 kDa. It plays an important role in the catabolism of active vitamin D metabolites particularly in the hydroxylation of calcitriol. This enzyme is located mainly in the kidney but also in other tissues such as the placenta and the pituitary gland. Its expression is regulated by various physiological factors including calcium and phosphate levels.
Biological function summary

This enzyme modulates the levels of active vitamin D influencing calcium and phosphate homeostasis. By converting calcitriol into its inactive forms CYP24A1 prevents excessive vitamin D activity which affects bone mineralization and cellular processes. It does not function as part of a larger complex but interacts closely with other vitamin D-metabolizing enzymes.

Pathways

CYP24A1 is an integral component of the vitamin D metabolism pathway. It acts in concert with another enzyme CYP27B1 which activates vitamin D by hydroxylating calcidiol to calcitriol. Together they balance the hormone levels vital for various cellular functions. The enzyme also participates in calcium signaling pathways affecting cellular growth and differentiation.

Dysregulation of CYP24A1 has been associated with conditions such as hypercalcemia and certain cancers like colorectal cancer. Overexpression or mutations in this enzyme lead to altered vitamin D metabolism exacerbating disease states. In cancer CYP24A1 often interplays with proteins like VDR the vitamin D receptor influencing cancer cell proliferation and response to therapy.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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