CYP24A1 KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 98%.
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Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 98%
Alternative names
1 25 dihydroxyvitamin D(3) 24 hydroxylase, 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial, 24-OHase, 25-dihydroxyvitamin D(3) 24-hydroxylase, CP 24, CP24A_HUMAN, CYP 24, Cytochrome P450 24A1, Cytochrome P450 24A1 mitochondrial, Cytochrome P450 family 24, Cytochrome P450 family 24 subfamily A member 1, Cytochrome P450 family 24 subfamily A polypeptide 1, Cytochrome P450 subfamily XXIV, Cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase), Cytochrome P450-CC24, EC 1.14.13.n4, Exo mitochondrial protein, HCAI, HCINF1, MGC126273, MGC126274, P450 CC24, Vitamin D 24 hydroxylase, Vitamin D(3) 24-hydroxylase, mitochondrial
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CYP24A1 KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 98%.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 98%
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- CYP24A1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CYP24A1 also known as cytochrome P450 family 24 subfamily A member 1 is an enzyme with a molecular mass of approximately 56 kDa. It plays an important role in the catabolism of active vitamin D metabolites particularly in the hydroxylation of calcitriol. This enzyme is located mainly in the kidney but also in other tissues such as the placenta and the pituitary gland. Its expression is regulated by various physiological factors including calcium and phosphate levels.
- Biological function summary
This enzyme modulates the levels of active vitamin D influencing calcium and phosphate homeostasis. By converting calcitriol into its inactive forms CYP24A1 prevents excessive vitamin D activity which affects bone mineralization and cellular processes. It does not function as part of a larger complex but interacts closely with other vitamin D-metabolizing enzymes.
- Pathways
CYP24A1 is an integral component of the vitamin D metabolism pathway. It acts in concert with another enzyme CYP27B1 which activates vitamin D by hydroxylating calcidiol to calcitriol. Together they balance the hormone levels vital for various cellular functions. The enzyme also participates in calcium signaling pathways affecting cellular growth and differentiation.
- Associated diseases and disorders
Dysregulation of CYP24A1 has been associated with conditions such as hypercalcemia and certain cancers like colorectal cancer. Overexpression or mutations in this enzyme lead to altered vitamin D metabolism exacerbating disease states. In cancer CYP24A1 often interplays with proteins like VDR the vitamin D receptor influencing cancer cell proliferation and response to therapy.
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Next Generation Sequencing - Human CYP24A1 knockout A549 cell lysate (ab273777)
Knockout achieved by CRISPR/Cas9; X = 1 bp deletion; Frameshift: 98%
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