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AB263163

Human CYSTM1 (ORF1 FL49) knockout HEK-293T cell lysate

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CYSTM1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon2.
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Sanger Sequencing - Human CYSTM1 (ORF1 FL49) knockout HEK-293T cell lysate (AB263163)
  • Sanger seq

Unknown

Sanger Sequencing - Human CYSTM1 (ORF1 FL49) knockout HEK-293T cell lysate (AB263163)

Homozygous : Insertion of the selection cassette in exon2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon2.

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Complementary Products

Cell Lines & Lysates

AB266448

Human CYSTM1 (ORF1 FL49) knockout HEK-293T cell line

Sanger Sequencing - Human CYSTM1 (ORF1 FL49) knockout HEK-293T cell line (AB266448)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
CYSTM1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ORF1 FL49 sometimes referred to in short as FL49 is an important component in biological processes at the cellular level. Structurally it has a molecular mass that has been experimentally determined to be approximately 55 kDa. Scientists have observed its expression in several tissues with notable presences in the liver and brain. This protein is a part of the greater ORF1 motif and is important to the individual cellular mechanisms where it is located.
Biological function summary

ORF1 FL49 has been shown to modulate various intracellular processes. It often functions in collaboration with a complex of other proteins to influence mRNA translation and stability. Researchers observed its involvement in regulating RNA processing where it affects both the synthesis and degradation of RNA. Studies have linked this protein to the energy metabolism pathways within the cell emphasizing its critical role in maintaining cellular homeostasis.

Pathways

Understanding ORF1 FL49 provides valuable insight into how cellular signaling and energy regulation occurs. This protein plays a significant part in the mTOR signaling pathway which affects cell growth and nutrient sensing. Furthermore it interacts with proteins such as S6K1 and AMPK integrating signals that influence metabolic activity and stress responses. This integration highlights the protein's contribution to maintaining energy balance within the organism.

Mutations or altered regulation of ORF1 FL49 have been associated with metabolic diseases and neurodegenerative conditions. Disruptions in the normal function of this protein can lead to abnormal cell growth and impaired energy management significantly contributing to disorders such as metabolic syndrome and Alzheimer's disease. In metabolic syndrome researchers have found that the protein's pathway interactions particularly with AMPK play a pivotal role in disease progression. In Alzheimer's for instance its relationship with mTOR signaling suggests that ORF1 FL49 may influence disease onset and progression particularly through its impact on neuronal cell health.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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