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AB257192

Human DAG1 knockout HEK-293T cell lysate

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DAG1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

View Alternative Names

156DAG, A3a, AGRNR, Agrin receptor, Alpha-DG, Beta-DG, Beta-dystroglycan, DAG, DAG1_HUMAN, Dystroglycan, Dystroglycan 1 (dystrophin-associated glycoprotein 1), Dystroglycan, alpha, Dystrophin-associated glycoprotein 1, MDDGC7, MDDGC9, OTTHUMP00000210857, OTTHUMP00000210858, alpha-DG-N

3 Images
Western blot - Human DAG1 knockout HEK-293T cell lysate (AB257192)
  • WB

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Western blot - Human DAG1 knockout HEK-293T cell lysate (AB257192)

Lane 1 : Wild-type HEK293T cell lysate (20 μg)

Lane 2 : DAG1 knockout HEK293T cell lysate (20 μg)

Lane 3 : Mouse heart tissue lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab62373 observed at 43 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab62373 Anti-beta Dystroglycan antibody [EP2200Y] was shown to specifically react with beta Dystroglycan in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266263 (knockout cell lysate ab257192) was used. Wild-type and beta Dystroglycan knockout samples were subjected to SDS-PAGE. ab62373 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (<a href='/en-us/products/primary-antibodies/beta-dystroglycan-antibody-ep2200y-ab62373'>ab62373</a>) at 1/500 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DAG1 knockout HEK293T cell lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Predicted band size: 97 kDa

Observed band size: 43 kDa

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Western blot - Human DAG1 knockout HEK-293T cell lysate (AB257192)
  • WB

Unknown

Western blot - Human DAG1 knockout HEK-293T cell lysate (AB257192)

Lane 1 : Wild-type HEK293T cell lysate (20 μg)

Lane 2 : DAG1 knockout HEK293T cell lysate (20 μg)

Lane 3 : Human skeletal muscle tissue lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab43125 observed at 43 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab43125 Anti-beta Dystroglycan antibody was shown to specifically react with beta Dystroglycan in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266263 (knockout cell lysate ab257192) was used. Wild-type and beta Dystroglycan knockout samples were subjected to SDS-PAGE. ab43125 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-beta Dystroglycan antibody (<a href='/en-us/products/primary-antibodies/beta-dystroglycan-antibody-ab43125'>ab43125</a>) at 1/500 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DAG1 knockout HEK293T cell lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Predicted band size: 97 kDa

Observed band size: 43 kDa

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Sanger Sequencing - Human DAG1 knockout HEK-293T cell lysate (AB257192)
  • Sanger seq

Unknown

Sanger Sequencing - Human DAG1 knockout HEK-293T cell lysate (AB257192)

Homozygous : 1 bp insertion in exon 2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
DAG1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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