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AB257407

Human DAPK3 (ZIP Kinase) knockout HEK-293T cell lysate

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DAPK3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

View Alternative Names

DAP kinase 3, DAP-like kinase, DAPK3_HUMAN, Death associated kinase 3, Death-associated protein kinase 3, Dlk, EC 2.7.11.1, FLJ36473, MYPT1 kinase, ZIP, ZIP kinase isoform, ZIP-kinase, ZIPK, zipper-interacting protein kinase

3 Images
Western blot - Human DAPK3 (ZIP Kinase) knockout HEK-293T cell lysate (AB257407)
  • WB

Lab

Western blot - Human DAPK3 (ZIP Kinase) knockout HEK-293T cell lysate (AB257407)

Lane 1 : Wild-type HEK-293T cell lysate (20µg)

Lane 2 : DAPK3 knockout HEK-293T cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab79422 observed at 53 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab79422 Anti-ZIP Kinase antibody [EPR1635] was shown to specifically react with ZIP Kinase in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line ab266755 (knockout cell lysate ab257407) lane below 53kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and ZIP Kinase knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab79422 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 5000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ZIP Kinase antibody [EPR1635] (<a href='/en-us/products/primary-antibodies/zip-kinase-antibody-epr1635-ab79422'>ab79422</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DAPK3 knockout HEK293T cell lysate at 20 µg

Predicted band size: 53 kDa

Observed band size: 53 kDa

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Western blot - Human DAPK3 (ZIP Kinase) knockout HEK-293T cell lysate (AB257407)
  • WB

Lab

Western blot - Human DAPK3 (ZIP Kinase) knockout HEK-293T cell lysate (AB257407)

Lane 1 : Wild-type HEK-293T cell lysate (20µg)

Lane 2 : DAPK3 knockout HEK-293T cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab51602 observed at 53 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab51602 Anti-ZIP Kinase antibody [EPR1636Y] was shown to specifically react with ZIP Kinase in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line ab266755 (knockout cell lysate ab257407) lane below 53kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and ZIP Kinase knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab51602 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ZIP Kinase antibody [EPR1636Y] (<a href='/en-us/products/primary-antibodies/zip-kinase-antibody-epr1636y-ab51602'>ab51602</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DAPK3 knockout HEK293T cell lysate at 20 µg

Predicted band size: 53 kDa

Observed band size: 53 kDa

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Sanger Sequencing - Human DAPK3 (ZIP Kinase) knockout HEK-293T cell lysate (AB257407)
  • Sanger seq

Unknown

Sanger Sequencing - Human DAPK3 (ZIP Kinase) knockout HEK-293T cell lysate (AB257407)

Homozygous : 1 bp insertion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.</p>" } } }
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Product details

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257407-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human DAPK3 knockout HEK293T cell lysate", "number":"AB257407-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB257407-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Gene name
DAPK3
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZIP Kinase also known as DAPK3 is a serine/threonine protein kinase with a molecular mass of approximately 52 kDa. It is expressed in a variety of tissues including the heart liver and skeletal muscle indicating its broad physiological importance. ZIP Kinase shows activity in phosphorylating specific substrates that influence cell structure and function playing a role in cellular processes.
Biological function summary

ZIP Kinase regulates apoptosis and autophagy impacting cell survival and homeostasis. It does not act in isolation but is part of a complex with other kinases and regulatory proteins. ZIP Kinase interacts with cytoskeletal proteins to mediate cell contraction and motility indicating its involvement in both normal cellular dynamics and stress responses.

Pathways

ZIP Kinase is involved in the apoptosis and autophagy pathways. Within the apoptosis pathway ZIP Kinase partners with proteins like DAPK1 influencing stress and injury responses. In the autophagy pathway it helps in regulating cellular degradation processes. These pathways allow ZIP Kinase to integrate signals that manage cell fate decisions collaboratively modulating cellular life and death.

ZIP Kinase associates with hypertension and certain types of cancer. In hypertension ZIP Kinase dysregulation may impact vascular smooth muscle cell contractility due to its connection with myosin light chain phosphorylation. In cancer altered ZIP Kinase activity may affect apoptosis linking it to aberrant cell proliferation. Its interaction with proteins like DAPK1 in both contexts underlines its role in pathological states where cell death and survival are disrupted.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB210528

Anti-ZIP Kinase antibody [EPR18809-86]

RabMAb|Recombinant|KO Validated|Trial Size

Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (AB210528)

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Cell Lines & Lysates

AB266755

Human DAPK3 (ZIP Kinase) knockout HEK-293T cell line

Advanced Validation

Western blot - Human DAPK3 (ZIP Kinase) knockout HEK-293T cell line (AB266755)

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Product promise

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For full details, please see our Terms & Conditions

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