Human DAXX knockout HeLa cell lysate
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DAXX KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon5 and 1 bp insertion in exon5.
View Alternative Names
BING 2, CENP-C binding protein, DAP 6, DAXX_HUMAN, Death associated protein 6, Death domain associated protein, Death domain-associated protein 6, EAP 1, ETS1-associated protein 1, Fas binding protein, Fas death domain-associated protein, MGC126245, MGC126246, hDaxx
- WB
Lab
Western blot - Human DAXX knockout HeLa cell lysate (AB257408)
Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : DAXX knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab32140 observed at 100 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32140 Recombinant Anti-Daxx antibody [E94] was shown to specifically react with Daxx in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265233 (knockout cell lysate ab257408) was used. Wild-type and Daxx knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32140 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Daxx antibody [E94] (<a href='/en-us/products/primary-antibodies/daxx-antibody-e94-ab32140'>ab32140</a>) at 1/5000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
DAXX knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human DAXX knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-daxx-knockout-hela-cell-line-ab265233'>ab265233</a>)
Predicted band size: 81 kDa
Observed band size: 100 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human DAXX knockout HeLa cell lysate (AB257408)
Allele-2 : 1 bp insertion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human DAXX knockout HeLa cell lysate (AB257408)
Allele-1 : 1 bp deletion in exon5
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-Daxx antibody [E94] (AB32140)](https://content.abcam.com/products/images/daxx-antibody-e94-ab32140--western-blot-img8782.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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