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AB263164

Human DCTPP1 (XTP3TPA) knockout HEK-293T cell lysate

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DCTPP1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
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Sanger Sequencing - Human DCTPP1 (XTP3TPA) knockout HEK-293T cell lysate (AB263164)
  • Sanger seq

Unknown

Sanger Sequencing - Human DCTPP1 (XTP3TPA) knockout HEK-293T cell lysate (AB263164)

Homozygous : Insertion of the selection cassette in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.

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Complementary Products

Primary Antibodies

AB172476

Anti-RRM2 antibody [EPR11820]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [EPR11820] (AB172476)

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Primary Antibodies

AB224051

Anti-XTP3TPA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XTP3TPA antibody (AB224051)

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Cell Lines & Lysates

AB266726

Human DCTPP1 (XTP3TPA) knockout HEK-293T cell line

Sanger Sequencing - Human DCTPP1 (XTP3TPA) knockout HEK-293T cell line (AB266726)

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Primary Antibodies

AB190698

Anti-XPB antibody [EPR14161]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-XPB antibody [EPR14161] (AB190698)

  • 5
  • 1
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
DCTPP1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XTP3TPA also known as Diecloropyrophosphatase is part of a larger family of proteins involved in pyrophosphate hydrolysis. This protein has a mass of approximately 65 kDa. XTP3TPA expression occurs in several tissues with higher levels in liver and kidney. These tissues play a major role in metabolic processes which would likely relate to this protein's functions.
Biological function summary

XTP3TPA functions to regulate cellular energy. These proteins also influence metabolic efficiency by hydrolyzing pyrophosphate to inorganic phosphate. XTP3TPA often operates within larger protein complexes to enhance its enzymatic role. This activity represents a common aspect of cellular metabolism impacting processes like ATP production.

Pathways

XTP3TPA integrates into the ATP synthesis and nucleotide metabolism pathways. Within these pathways XTP3TPA sometimes collaborates with proteins such as ATP synthase coordinating energy production and utilization. Its presence in these pathways ensures proper energy allocation which is important for cellular survival and function.

XTP3TPA abnormalities link to metabolic disorders such as type 2 diabetes and metabolic syndrome. Disrupted function or expression of XTP3TPA might impact proteins like AMP-activated protein kinase (AMPK). This disruption can lead to metabolic imbalances necessitating more research into XTP3TPA for potential therapeutic targets.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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