Human DDB2 knockout HeLa cell lysate
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DDB2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 29 bp deletion in exon1 and 2 bp insertion in exon1.
View Alternative Names
DDB p48 subunit, DDB2_HUMAN, DDBb, DNA damage-binding protein 2, Damage-specific DNA-binding protein 2, UV-DDB 2, UV-damaged DNA-binding protein 2, Xeroderma pigmentosum group E protei
- WB
Unknown
Western blot - Human DDB2 knockout HeLa cell lysate (AB257177)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : DDB2 knockout HeLa cell lysate (20 μg)
Lane 3 : Raji cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab181136 observed at 48 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab181136 Anti-DDB2 antibody [EPR9811] was shown to specifically react with DDB2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265115 (knockout cell lysate ab257177) was used. Wild-type and DDB2 knockout samples were subjected to SDS-PAGE. ab181136 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-DDB2 antibody [EPR9811] (<a href='/en-us/products/primary-antibodies/ddb2-antibody-epr9811-ab181136'>ab181136</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
DDB2 knockout HeLa cell lysate at 20 µg
Lane 3:
Raji cell lysate at 20 µg
Predicted band size: 48 kDa
Observed band size: 48 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human DDB2 knockout HeLa cell lysate (AB257177)
Allele-1 : 29 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human DDB2 knockout HeLa cell lysate (AB257177)
Allele-2 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human DDB2 knockout HeLa cell lysate (AB257177)
Allele-3 : 2 bp insertion in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Engagement in DNA repair mechanisms allows DDB2 to help maintain genomic stability. It forms a critical part of the DDB1-DDB2 complex which collaborates with other proteins in the initial damage recognition step of NER. This involvement ensures the repair of UV-induced damage and bulky DNA adducts preventing mutations that can cause harmful genetic alterations.
Pathways
The involvement of DDB2 is important within the nucleotide excision repair and transcription-coupled repair pathways. In these pathways DDB2 partners with proteins such as XPC and XPA facilitating the recognition and verification of DNA damage. These interactions ensure an accurate and efficient repair process protecting cells from potential genotoxic stress.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB2 antibody [EPR9811] (AB181136)](https://content.abcam.com/products/images/ddb2-antibody-epr9811-ab181136--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img180670.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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