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AB261660

Human DDX17 knockout HEK-293 cell lysate

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DDX17 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion.
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Western blot - Human DDX17 knockout HEK-293 cell lysate (AB261660)
  • WB

Lab

Western blot - Human DDX17 knockout HEK-293 cell lysate (AB261660)

Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2 : DDX17 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lanes 1 - 3 : Merged signal (red and green). Green - ab71958 observed at 72, 85 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab71958 was shown to recognize DDX17 in wild-type HEK-293 cells as signal was lost at the expected MW in DDX17 knockout cell line ab261721 (knockout cell lysate ab261660). Additional cross-reactive bands were observed in the wild-type and knockout samples. Wild-type and DDX17 knockout samples were subjected to SDS-PAGE. ab71958 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DDX17 antibody [2248C2a] (<a href='/en-us/products/primary-antibodies/ddx17-antibody-2248c2a-ab71958'>ab71958</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 whole cell lysate at 20 µg

Lane 2:

DDX17 knockout HEK-293 whole cell lysate at 20 µg

Lane 2:

Western blot - Human DDX17 knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-ddx17-knockout-hek-293-cell-line-ab261721'>ab261721</a>)

Lane 3:

HeLa whole cell lysate at 20 µg

Predicted band size: 72 kDa

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Western blot - Human DDX17 knockout HEK-293 cell lysate (AB261660)
  • WB

Lab

Western blot - Human DDX17 knockout HEK-293 cell lysate (AB261660)

Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : DDX17 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lanes 1 - 3 : Merged signal (red and green). Green - ab24601 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab24601 was shown to recognize DDX17 in wild-type HEK-293 cells as signal was lost at the expected MW in DDX17 knockout cell line ab261721 (knockout cell lysate ab261660). Additional cross-reactive bands were observed in the wild-type and knockout cell lysate. Wild-type and DDX17 knockout samples were subjected to SDS-PAGE. ab24601 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DDX17 antibody (<a href='/en-us/products/primary-antibodies/ddx17-antibody-ab24601'>ab24601</a>) at 1 µg/mL

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

DDX17 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human DDX17 knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-ddx17-knockout-hek-293-cell-line-ab261721'>ab261721</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 72 kDa

false

Western blot - Human DDX17 knockout HEK-293 cell lysate (AB261660)
  • WB

Lab

Western blot - Human DDX17 knockout HEK-293 cell lysate (AB261660)

Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : DDX17 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lanes 1 - 3 : Merged signal (red and green). Green - ab180190 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab180190 was shown to recognize DDX17 in wild-type HEK-293 cells as signal was lost at the expected MW in DDX17 knockout cell line ab261721 (knockout cell lysate ab261660). Additional cross-reactive bands were observed in the wild-type and knockout cell lysate. Wild-type and DDX17 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab180190 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DDX17 antibody [EPR13807(B)] (<a href='/en-us/products/primary-antibodies/ddx17-antibody-epr13807b-ab180190'>ab180190</a>) at 1/5000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

DDX17 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human DDX17 knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-ddx17-knockout-hek-293-cell-line-ab261721'>ab261721</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 72 kDa

false

Key facts

Cell type

HEK-293

Species or organism

Human

Tissue

Kidney

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9 X = 1 bp insertion

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Complementary Products

Cell Lines & Lysates

AB261721

Human DDX17 knockout HEK-293 cell line

Advanced Validation

Western blot - Human DDX17 knockout HEK-293 cell line (AB261721)

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Primary Antibodies

AB180190

Anti-DDX17 antibody [EPR13807(B)]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX17 antibody [EPR13807(B)] (AB180190)

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Proteins & Peptides

AB78685

Recombinant Human PAK4 protein

SDS-PAGE - Recombinant Human PAK4 protein (AB78685)

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Cell Lines & Lysates

AB258005

Human IPO4 (Importin4/Imp4) knockout HEK-293T cell lysate

Western blot - Human IPO4 (Importin4/Imp4) knockout HEK-293T cell lysate (AB258005)

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
DDX17
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX17 also known as RNA helicase p72 is an enzyme that unwinds RNA molecules. It belongs to the DEAD-box protein family. Its mechanical action involves utilizing ATP to unwind RNA facilitating various RNA processing events. DDX17 weighs approximately 73 kDa making it a relatively large protein. The enzyme expresses in multiple tissues but shows substantial activity in HEK 293 cells a human embryonic kidney cell line.
Biological function summary

This enzyme plays a significant role in RNA metabolism. DDX17 is part of the spliceosome complex which is essential for splicing pre-mRNA into mature mRNA. Through its helicase activity DDX17 ensures the proper remodeling of RNA structures which is necessary for accurate splicing. The protein also contributes to the regulation of gene expression and has been observed to influence transcription factors.

Pathways

RNA processing and maturation are key biological functions of DDX17. The protein integrates into the mRNA splicing pathway where it orchestrates proper RNA folding and splice site selection. Furthermore DDX17 interacts with other DEAD-box proteins such as DDX5 to assist in chromatin remodeling and transcription regulation facilitating efficient gene expression.

Abnormal DDX17 expression or mutation links to certain cancers including breast cancer and leukemia. The protein along with DDX5 affects tumor progression by regulating pathways involved in cell proliferation. These pathways and protein interactions highlight the importance of DDX17 in maintaining normal cell function and its potential as a therapeutic target in cancer treatment.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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