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AB263165

Human DENND4B knockout HeLa cell lysate

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DENND4B KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon10 and 1 bp insertion in exon10.
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Sanger Sequencing - Human DENND4B knockout HeLa cell lysate (AB263165)
  • Sanger seq

Unknown

Sanger Sequencing - Human DENND4B knockout HeLa cell lysate (AB263165)

Allele-1 : 1 bp deletion in exon10

Sanger Sequencing - Human DENND4B knockout HeLa cell lysate (AB263165)
  • Sanger seq

Unknown

Sanger Sequencing - Human DENND4B knockout HeLa cell lysate (AB263165)

Allele-2 : 1 bp insertion in exon10

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon10 and 1 bp insertion in exon10.

Disease

Adenocarcinoma

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Complementary Products

Cell Lines & Lysates

AB265967

Human DENND4B knockout HeLa cell line

Sanger Sequencing - Human DENND4B knockout HeLa cell line (AB265967)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
DENND4B
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DENND4B also known as the DENN domain-containing protein 4B plays a role in the regulation of endosomal trafficking. It weighs approximately 160 kDa and acts as a guanine nucleotide exchange factor (GEF) for small GTPases. This protein shows expression across various tissues with heightened levels in the brain and testis. DENND4B is associated with cellular processes requiring membrane fusion and trafficking.
Biological function summary

DENND4B influences endocytosis and membrane transport by activating the Rab family of GTPases. This protein contributes to the coordination of membrane dynamics involved in intracellular transport systems. Although not typically part of a large complex DENND4B interacts with several proteins that affect endosomal sorting and trafficking therefore aiding cellular recycling and degradation of receptors and other membrane proteins.

Pathways

DENND4B plays a role in the endosomal trafficking pathway particularly affecting the recycling pathway of GTPase Rab10. It also interplays within the mTOR signaling pathway influencing autophagy processes. Proteins such as Rab8 and Rab4 link to DENND4B in these pathways reflecting its impact on cellular growth and nutrient response mechanisms.

DENND4B has connections to neurological disorders and certain cancers. Abnormal expression or mutations in DENND4B can lead to disruptions in cellular trafficking contributing to conditions like neurodegeneration. In relation to cancer alterations in DENND4B affect cell proliferation and can influence cancer progression. The protein's interaction with Rab GTPases particularly Rab10 further supports its involvement in these disease mechanisms.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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