DENND4B KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon10 and 1 bp insertion in exon10.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon10 and 1 bp insertion in exon10.
Alternative names
DEN4B_HUMAN, DENN domain-containing protein 4B, DENN/MADD domain containing 4B, Dennd4b, KIAA0476, hCG_18235
What's included?
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DENND4B KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon10 and 1 bp insertion in exon10.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon10 and 1 bp insertion in exon10.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- DENND4B
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DENND4B also known as the DENN domain-containing protein 4B plays a role in the regulation of endosomal trafficking. It weighs approximately 160 kDa and acts as a guanine nucleotide exchange factor (GEF) for small GTPases. This protein shows expression across various tissues with heightened levels in the brain and testis. DENND4B is associated with cellular processes requiring membrane fusion and trafficking.
- Biological function summary
DENND4B influences endocytosis and membrane transport by activating the Rab family of GTPases. This protein contributes to the coordination of membrane dynamics involved in intracellular transport systems. Although not typically part of a large complex DENND4B interacts with several proteins that affect endosomal sorting and trafficking therefore aiding cellular recycling and degradation of receptors and other membrane proteins.
- Pathways
DENND4B plays a role in the endosomal trafficking pathway particularly affecting the recycling pathway of GTPase Rab10. It also interplays within the mTOR signaling pathway influencing autophagy processes. Proteins such as Rab8 and Rab4 link to DENND4B in these pathways reflecting its impact on cellular growth and nutrient response mechanisms.
- Associated diseases and disorders
DENND4B has connections to neurological disorders and certain cancers. Abnormal expression or mutations in DENND4B can lead to disruptions in cellular trafficking contributing to conditions like neurodegeneration. In relation to cancer alterations in DENND4B affect cell proliferation and can influence cancer progression. The protein's interaction with Rab GTPases particularly Rab10 further supports its involvement in these disease mechanisms.
Product promise
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2 product images
Sanger Sequencing - Human DENND4B knockout HeLa cell lysate (ab263165)
Allele-1: 1 bp deletion in exon10
Sanger Sequencing - Human DENND4B knockout HeLa cell lysate (ab263165)
Allele-2: 1 bp insertion in exon10
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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