Human DENND5A knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human DENND5A knockout HeLa cell lysate (AB258392)
Allele-2 : 2 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human DENND5A knockout HeLa cell lysate (AB258392)
Allele-1 : 8 bp deletion in exon2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DENND5A influences intracellular signaling and membrane trafficking. It does this by activating Rab protein family members essential for vesicle formation movement and fusion. DENND5A participates in endocytic pathways which involves clathrin-mediated endocytosis a pathway critical for material uptake into cells. There is no clear evidence yet whether DENND5A associates within larger protein complexes but its interaction with Rab proteins suggests close collaboration in cellular trafficking tasks.
Pathways
DENND5A integrates into the membrane trafficking and cellular communication networks. It plays an important role in the endocytosis pathway and syncs with proteins like Rab22A facilitating efficient vesicular transport from the plasma membrane. Another significant pathway is the mannose 6-phosphate receptor pathway where DENND5A influences lysosomal enzyme trafficking vital for cell maintenance.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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