Human DEPDC5 knockout A549 cell lysate
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DEPDC5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon12.
View Alternative Names
DEP domain containing 5, DEP domain-containing protein 5, DEP.5, DEPD5_HUMAN, FFEVF, KIAA0645
- WB
Lab
Western blot - Human DEPDC5 knockout A549 cell lysate (AB258394)
Lane 1 : Wild-type A549 cell lysate 20 μg
Lane 2 : DEPDC5 knockout A549 cell lysate 20 μg
Lane 3 : Human Brain cell lysate 20 μg
Lane 4 : Mouse Brain cell lysate 20 μg
Lane 5 : PC-3 cell lysate 20 μg
False colour image of Western blot : Anti-DEPDC5 antibody [EPR20497-23] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab213181 was shown to bind specifically to DEPDC5. A band was observed at 181 kDa in wild-type A549 cell lysates with no signal observed at this size in DEPDC5 knockout cell line ab266906 (knockout cell lysate ab258394). To generate this image, wild-type and DEPDC5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-DEPDC5 antibody [EPR20497-23] (<a href='/en-us/products/primary-antibodies/depdc5-antibody-epr20497-23-ab213181'>ab213181</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
DEPDC5 knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human DEPDC5 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-depdc5-knockout-a549-cell-line-ab266906'>ab266906</a>)
Lane 3:
Human brain tissue lysate at 20 µg
Lane 4:
Mouse brain tissue lysate at 20 µg
Predicted band size: 178 kDa
Observed band size: 181 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human DEPDC5 knockout A549 cell lysate (AB258394)
Homozygous : 1 bp insertion in exon12
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DEPDC5 acts as a negative regulator of the mTORC1 signaling pathway by being a part of the GATOR1 complex. This complex is involved in nutrient sensing and regulation of cell growth and metabolism. DEPDC5’s role in mTORC1 regulation links it to the control of protein synthesis and autophagy essential processes in maintaining cellular homeostasis. By interacting with other components of the GATOR1 complex DEPDC5 contributes not only to inhibiting mTORC1 activity but also to the broader metabolic balance.
Pathways
DEPDC5 is integral to the mTOR signaling pathway and the amino acid sensing pathways. The protein acts in coordination with other GATOR1 complex components such as NPRL2 and NPRL3 which together monitor nutrient availability and adapt cellular functions accordingly. These pathways highlight DEPDC5’s role in connecting nutrient signals to cellular growth responses showing its influence on critical regulatory networks affecting cell growth and proliferation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
![Western blot - Anti-DEPDC5 antibody [EPR20497-23] (AB213181)](https://content.abcam.com/products/images/depdc5-antibody-epr20497-23-ab213181--western-blot-img29227.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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