Human DGUOK (dGK) knockout HEK-293T cell lysate
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DGUOK KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
View Alternative Names
Deoxyguanosine kinase mitochondrial, dGK
- Sanger seq
Unknown
Sanger Sequencing - Human DGUOK (dGK) knockout HEK-293T cell lysate (AB258843)
Homozygous : 1 bp insertion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Deoxyguanosine kinase contributes significantly to the maintenance of mitochondrial DNA by ensuring the supply of deoxyribonucleotide triphosphates (dNTPs). It does not function as part of a larger protein complex working independently to convert purine deoxynucleosides in a rate-limiting step. The enzyme ensures that cellular processes requiring DNA replication and repair can proceed highlighting its importance in cellular functionality and homeostasis.
Pathways
Deoxyguanosine kinase participates in the nucleotide salvage pathway which is pivotal for recycling nucleosides into nucleotide pools. This pathway complements the de novo synthesis pathway providing a rapid response mechanism for nucleotide demand. dGK operates alongside other kinases like thymidine kinase which similarly act to phosphorylate nucleosides. This interconnection facilitates the balance of nucleotide pools within the cell important for effective DNA synthesis and repair.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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