Human DHCR24 knockout A-431 cell lysate
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DHCR24 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 2 bp deletion Frameshift: 99.61%.
View Alternative Names
24-dehydrocholesterol reductase, 3-beta-hydroxysterol delta-24-reductase, DCE, DHC24_HUMAN, Delta(24)-sterol reductase, Desmosterol to cholesterol enzyme, Dhcr24, Diminuto dwarf1 homolog, Diminuto/dwarf1 homolog, KIAA0018, Nbla03646, Seladin-1, Selective AD indicator 1, Selective Alzheimer's Disease Indicator 1
- WB
Lab
Western blot - Human DHCR24 knockout A-431 cell lysate (AB263920)
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : DHCR24 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lanes 1 - 3 : Merged signal (red and green). Green - ab181062 observed at 51 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab181062 was shown to react with DHCR24 in wild-type A-431 cells in Western blot Loss of signal was observed when DHCR24 knockout cell line ab262480 (knockout cell lysate ab263920) was used. Wild-type A-431 and DHCR24 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab181062 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Seladin 1 antibody [EPR10743] (<a href='/en-us/products/primary-antibodies/seladin-1-antibody-epr10743-ab181062'>ab181062</a>) at 1/2000 dilution
Lane 1:
Wild-type A431 whole cell lysate at 20 µg
Lane 2:
DHCR24 knockout A431 whole cell lysate at 20 µg
Lane 2:
Western blot - Human DHCR24 knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-dhcr24-knockout-a-431-cell-line-ab262480'>ab262480</a>)
Lane 3:
HepG2 whole cell lysate at 20 µg
Predicted band size: 60 kDa
Observed band size: 51 kDa
false
- NGS
Supplier Data
Next Generation Sequencing - Human DHCR24 knockout A-431 cell lysate (AB263920)
Knockout achieved by CRISPR/Cas9; X = 2 bp deletion; Frameshift : 99.61%
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Seladin 1 plays an important role in cellular processes particularly in response to oxidative stress. The protein is not part of a larger complex but functions independently to reduce highly reactive oxygen species levels in cells. It helps in maintaining cellular integrity and contributes to the survival of neuronal cells under stress conditions. Additionally it provides resistance to oxidative stress-induced apoptosis by modulating intracellular antioxidative defense mechanisms.
Pathways
Seladin 1 plays an integral role in the cholesterol biosynthesis pathway and is closely associated with the mevalonate pathway. Its activity directly influences the availability of cholesterol and other sterols impacting membrane fluidity and cellular functions dependent on sterol concentrations. The enzyme interacts with related proteins such as HMG-CoA reductase an important regulatory enzyme within the cholesterol synthesis pathway. These interactions ensure that cellular cholesterol levels are well-regulated to meet physiological demands.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB314222
Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free
BOND RX™ Validated|RabMAb|KO Validated|Recombinant
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- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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