Human DHRS13 knockout HeLa cell lysate
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DHRS13 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.
View Alternative Names
DHRS 13, Dehydrogenase/reductase (SDR family) member 13, Dehydrogenase/reductase SDR family member 13, MGC23280, OTTHUMP00000163522, SDR7C5, Short chain dehydrogenase/reductase family 7C member 5
- Sanger seq
Unknown
Sanger Sequencing - Human DHRS13 knockout HeLa cell lysate (AB263170)
Homozygous : 1 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The activities of DHRS13 involve critical processes related to metabolic regulation and energy homeostasis. The enzyme plays a role in lipid biosynthesis regulating fatty acid and steroid activities. Although no evidence suggests DHRS13 forms part of a larger protein complex it exerts influence on numerous cellular activities through its enzymatic actions. Despite not forming a complex it interacts indirectly with substrates and co-factors necessary for proper metabolic functioning.
Pathways
DHRS13 integrates into cellular metabolic pathways impacting several biochemical processes. In the lipid metabolism pathway it interacts with proteins such as DHRS7 and DHRS12 contributing to the regulation of fatty acid oxidation and lipid homeostasis. Another important pathway is the NADP-dependent oxidative pathway where DHRS13's enzymatic capacity assists in balancing cellular oxidation-reduction states providing stability for metabolic controls within cells.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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