Human DHX58 (RLR) knockout A549 cell lysate
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DHX58 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and 2 bp deletion in exon4.
View Alternative Names
D11LGP2, D11lgp2e, DEXH (Asp Glu X His) box polypeptide 58, DEXH box polypeptide 58, DHX58_HUMAN, LGP 2, Ortholog of mouse D11lgp2, Probable ATP-dependent RNA helicase DHX58, Probable ATP-dependent helicase LGP2, Protein D11Lgp2 homolog, RIG-I-like receptor LGP2, RLR, RNA helicase LGP2
- Sanger seq
Unknown
Sanger Sequencing - Human DHX58 (RLR) knockout A549 cell lysate (AB257921)
Allele-1 : 2 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human DHX58 (RLR) knockout A549 cell lysate (AB257921)
Allele-2 : 1 bp deletion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DHX58 functions as a regulatory molecule in innate immunity. It usually does not act alone and can form complexes with other RLRs like RIG-I and MDA5. These complexes enhance antiviral signal transduction by modulating downstream components of the interferon signaling pathway. By influencing these pathways DHX58 contributes to the regulation of the immune response ensuring the proper activation of antiviral defense mechanisms.
Pathways
Several immune pathways involve DHX58 namely the RIG-I-like receptor signaling pathway and the interferon regulatory pathways. These pathways are critical for maintaining the body's defense against viral infections. Within these pathways DHX58 interacts with related proteins such as RIG-I and MDA5 which are important for recognizing viral RNA and facilitating subsequent immune responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
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Product promise
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