Human DNAJB1 (Hsp40) knockout HEK-293T cell lysate
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DNAJB1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 29 bp deletion in exon 1.
View Alternative Names
DNAJ 1, DNAJ B1, DNAJB1 protein, DNJB1_HUMAN, DnaJ (Hsp40) homolog subfmaily B member 1, DnaJ heat shock protein family (Hsp40) member B1, DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, HDJ-1, HSPF 1, Heat shock 40 kDa protein 1, Heat shock 40kD protein 1, Heat shock protein 40, Hsp 40, Human DnaJ protein 1, RSPH16B, Radial spoke 16 homolog B, Sis1
- WB
Unknown
Western blot - Human DNAJB1 (Hsp40) knockout HEK-293T cell lysate (AB258400)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : DNAJB1 knockout HEK293T cell lysate (20 ug)
Lane 3 : A549 cell lysate (20 ug)
Lane 4 : Daudi cell lysate (20 ug)
ab223607 was shown to specifically react with Hsp40 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266344 (knockout cell lysate ab258400) was used. Wild-type and Hsp40 knockout samples were subjected to SDS-PAGE. ab223607 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
false
- Sanger seq
Unknown
Sanger Sequencing - Human DNAJB1 (Hsp40) knockout HEK-293T cell lysate (AB258400)
Homozygous : 29 bp deletion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Hsp40 proteins participate in cell signaling and stress response by modulating the activity of Hsp70 forming a functional complex with it to guide protein folding processes. This process is essential for protein quality control and cellular protection. It interacts with unfolded proteins and delivers them to Hsp70 enhancing its ATPase activity necessary for the proper functioning of these molecular chaperones. The expression of Hsp40 in stress conditions highlights its importance in the protein quality control system.
Pathways
Hsp40 is involved in important cellular mechanisms like the unfolded protein response and protein catabolism pathways. It works closely with Hsp70 playing a pivotal role in managing protein misfolding and aggregation vital for cellular stress response and recovery. In these pathways Hsp40's function ensures the proper folding and degradation of damaged proteins maintaining proteostasis in the cell.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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