Human DNAJC13 (RME-8) knockout HeLa cell lysate
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DNAJC13 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon15 and 2 bp deletion in exon15.
View Alternative Names
DJC13_HUMAN, DNAJC13, DnaJ (Hsp40) homolog subfamily C member 13, DnaJ domain containing protein RME 8, DnaJ homolog subfamily C member 13, KIAA0678, RME-8, Required for receptor-mediated endocytosis 8
- Sanger seq
Unknown
Sanger Sequencing - Human DNAJC13 (RME-8) knockout HeLa cell lysate (AB258847)
Allele-1 : 2 bp deletion in exon15
- Sanger seq
Unknown
Sanger Sequencing - Human DNAJC13 (RME-8) knockout HeLa cell lysate (AB258847)
Allele-2 : 1 bp insertion in exon15
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The activity of RME-8 is essential for the maintenance of cellular homeostasis. It interacts with other proteins to form a complex that facilitates proper protein sorting within the endosomal network. RME-8's role in this intricate system ensures the appropriate recycling of receptors and disposal of damaged cellular components aiding in overall cell function. The protein regulates the spatial organization of endosomes an important process necessary for nutrient uptake and receptor recycling which impacts cell signaling and metabolism.
Pathways
RME-8 participates in endocytic pathways where it collaborates with proteins like Eps15 and Hsc70 to manage cargo trafficking. The endocytic pathway heavily relies on RME-8's functions for sustaining efficient internalization and routing of molecules such as receptors and ligands. Additionally the protein interacts with components of the retromer complex indicating a connection to the retromer-mediated transport pathway. This involvement may have effects on intracellular transport activities influencing the broader cellular communication network.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB265622
Human CDC42BPA (CDC42 binding protein kinase alpha) knockout HeLa cell line
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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