Human DNAJC9 knockout HEK-293T cell lysate
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DNAJC9 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 157 bp deletion in exon 1.
View Alternative Names
DNJC9_HUMAN, DnaJ Hsp40 homolog subfamily C member 9, DnaJ homolog subfamily C member 9, DnaJ protein SB73, HDJC9, J DOMAIN OF DNAJ LIKE PROTEIN 1, JDD1, SB73
- WB
Lab
Western blot - Human DNAJC9 knockout HEK-293T cell lysate (AB257926)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : DNAJC9 knockout HEK-293T cell lysate 20 μg
Lane 3 : PC-3 cell lysate 20 μg
Lane 4 : HepG2 cell lysate 20 μg
False colour image of Western blot : Anti-DNAJC9 antibody [EPR9857] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab150394 was shown to bind specifically to DNAJC9. A band was observed at 35 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in DNAJC9 knockout cell line ab266364 (knockout cell lysate ab257926). The band observed in the knockout lysate lane below 35 kDa is likely to represent a truncated form of DNAJC9. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and DNAJC9 knockout HEK-293T cell lysates were analysed.First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
false
- WB
Lab
Western blot - Human DNAJC9 knockout HEK-293T cell lysate (AB257926)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : DNAJC9 knockout HEK-293T cell lysate 20 μg
Lane 3 : PC-3 cell lysate 20 μg
Lane 4 : HepG2 cell lysate 20 μg
False colour image of Western blot : Anti-DNAJC9 antibody [EPR9856] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab166612 was shown to bind specifically to DNAJC9. A band was observed at 35 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in DNAJC9 knockout cell line ab266364 (knockout cell lysate ab257926). The band observed in the knockout lysate lane below 35 kDa is likely to represent a truncated form of DNAJC9. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and DNAJC9 knockout HEK-293T cell lysates were analysed.First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
false
- Sanger seq
Unknown
Sanger Sequencing - Human DNAJC9 knockout HEK-293T cell lysate (AB257926)
Homozygous : 157 bp deletion in exon 1
Reactivity data
Product details
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Immunocytochemistry/ Immunofluorescence - Anti-DNAJC9 antibody [EPR9857] (AB150394)](https://content.abcam.com/products/images/dnajc9-antibody-epr9857-ab150394--immunocytochemistry-immunofluorescence-img1915.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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