JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB263174

Human DPH2 knockout HeLa cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

DPH2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon2 and 7 bp deletion in exon2.
2 Images
Sanger Sequencing - Human DPH2 knockout HeLa cell lysate (AB263174)
  • Sanger seq

Unknown

Sanger Sequencing - Human DPH2 knockout HeLa cell lysate (AB263174)

Allele-1 : 20 bp deletion in exon2

Sanger Sequencing - Human DPH2 knockout HeLa cell lysate (AB263174)
  • Sanger seq

Unknown

Sanger Sequencing - Human DPH2 knockout HeLa cell lysate (AB263174)

Allele-2 : 7 bp deletion in exon2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon2 and 7 bp deletion in exon2.

Disease

Adenocarcinoma

style
left-column

Complementary Products

Cell Lines & Lysates

AB265657

Human DPH2 knockout HeLa cell line

Sanger Sequencing - Human DPH2 knockout HeLa cell line (AB265657)

  • 0
  • 0
Proteins & Peptides

AB158306

Recombinant Human DPH2 protein

SDS-PAGE - Recombinant Human DPH2 protein (AB158306)

  • 0
  • 0
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263174-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human DPH2 knockout HeLa cell lysate", "number":"AB263174-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB263174-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
DPH2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPH2 also known as diphthamide biosynthesis protein 2 functions mechanically in catalyzing the first step of diphthamide biosynthesis. The protein incorporates a unique post-translational modification into histidine residues on elongation factor 2 (EEF2). DPH2 weighs approximately 53 kDa and is expressed in various tissues with high levels seen in the liver and testis. Its structural genes reside on human chromosome 17.
Biological function summary

Diphthamide biosynthesis is important to protein synthesis and cellular resilience against bacterial toxins like diphtheria toxin. DPH2 operates within a multiprotein complex including DPH1 and DPH3 necessary for EEF2 modification. Proper function of DPH2 ensures that EEF2 maintains its role in translational fidelity which is indispensable for normal cellular processes.

Pathways

Diphthamide biosynthesis connects to the protein synthesis and modification pathway. DPH2's activities integrate seamlessly with the translational machinery specifically influencing EEF2's function in the ribosome. In addition DPH2 works with DPH1 DPH3 and other proteins in the biosynthesis pathway to fulfill its role efficiently supporting cellular defense mechanisms against translational errors induced by toxins.

Defects in DPH2 relate to cancer and neurological conditions. Loss of function mutations in DPH2 can affect cellular processes potentially contributing to tumorigenesis where DPH1 is another connected protein of interest. Genetic alterations in DPH2 can also interfere with neural cell proliferation and survival linking to certain neurodegenerative disorders and emphasizing its role in maintaining cell health.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com