Human DPM2 knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
DPM2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2 and 4 bp deletion in exon2.
View Alternative Names
Dolichol phosphate (beta D) mannosyltransferase 2, Dolichol phosphate mannose biosynthesis regulatory protein, Dolichol phosphate mannose synthase 2, Dolichyl phosphate mannosyltransferase 2, Dolichyl phosphate mannosyltransferase polypeptide 2, regulatory subunit, R75484, RP23 255P20.5
- Sanger seq
Unknown
Sanger Sequencing - Human DPM2 knockout HeLa cell lysate (AB263175)
Allele-1 : 4 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human DPM2 knockout HeLa cell lysate (AB263175)
Allele-2 : 2 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DPM2 is involved in the assembly of the glycosylation precursor dolichol-phosphate-mannose which is critical for the proper synthesis of glycoproteins. As part of the DPM synthase complex DPM2 teams with the catalytic subunit DPM1 and the anchoring subunit DPM3. These interactions ensure effective glycosylation necessary for protein folding and function. The glycosylation process is essential for the stability and activity of numerous proteins within eukaryotic organisms.
Pathways
DPM2 plays a central role in the protein glycosylation pathway and the synthesis of glycosylphosphatidylinositol (GPI) anchors. It works closely with DPM1 activating it to transfer mannose molecules from GDP-mannose to dolichol-phosphate an important step in the formation of GPI-anchored proteins. These pathways are vital for cell surface expression and protein localization implicating DPM2 in broader cell signaling and communication networks.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com