Human DR1 knockout HEK-293T cell lysate
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DR1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
View Alternative Names
Down regulator of transcription 1 TBP binding, Down-regulator of transcription 1, Dr1 protein, NC 2, NC2-beta, NC2B_HUMAN, Negative co factor 2, Negative cofactor 2-beta, Protein Dr1, TATA box/binding protein associated phosphoprotein DR1, TATA-binding protein-associated phosphoprotein, down-regulator of transcription 1, TBP-binding (negative cofactor 2)
- Sanger seq
Unknown
Sanger Sequencing - Human DR1 knockout HEK-293T cell lysate (AB257927)
Allele-2 : Insertion of the selection cassette in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human DR1 knockout HEK-293T cell lysate (AB257927)
Allele-1 : 10 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DR1 plays a critical role in chromatin remodeling and transcription regulation. It is a part of the DR1/DRAP1 complex which influences the basal transcription machinery. This complex modulates transcription from TATA-containing promoters by altering the TBP-induced DNA bending leading to changes in gene expression patterns that are necessary for cellular homeostasis and development. Its interaction with other transcriptional modifiers helps to finely tune the transcriptional responses of cells.
Pathways
DR1 is integral to the transcriptional regulation pathway specifically affecting the process of transcription initiation. The DR1/DRAP1 complex plays an essential role in maintaining the balance between activators and repressors at the promoter sites. It works closely with proteins like TBP-associated factors (TAFs) which are part of the transcription factor IID (TFIID) complex. This ensures that transcription is controlled precisely allowing cells to respond accurately to various signaling cues.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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