Human DRG1 knockout HEK-293T cell lysate
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DRG1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 1.
View Alternative Names
DRG-1, Translation factor GTPase DRG1, NEDD-3, TRAFAC GTPase DRG1, Developmentally-regulated GTP-binding protein 1, Neural precursor cell expressed developmentally down-regulated protein 3, NEDD3, DRG1
- Sanger seq
Unknown
Sanger Sequencing - Human DRG1 knockout HEK-293T cell lysate (AB257928)
Homozygous : 5 bp deletion in exon 1
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Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DRG1 engages in several critical cellular processes beyond its mechanical functions. One of its notable roles involves contributions to ribosome biogenesis although it does not form a permanent complex with the ribosome. The protein supports cellular growth and division highlighting its potential in affecting cell cycle regulation. DRG1 associates transiently with other proteins allowing it to influence a range of biological functions including cellular growth signaling pathways.
Pathways
Research indicates that DRG1 links to important regulatory networks such as the Ras signaling and translational control pathways. Within these pathways DRG1 interacts with proteins like eukaryotic initiation factors which are important for translation regulation. This interaction plays a role in coordinating protein synthesis which supports overall cellular growth and response to environmental cues. Understanding its place in these pathways sheds light on how cells control protein production and growth.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com