Human DVL2 knockout A549 cell lysate
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- WB
Lab
Western blot - Human DVL2 knockout A549 cell lysate (AB288317)
Lane 1 : Wild-type A549 cell lysate 20 μg
Lane 2 : dvl2 knockout A549 cell lysate 20 μg
Lane 3 : HepG2 cell lysate 20 μg
Lane 4 : THP-1 cell lysate 20 μg
False colour image of Western blot : Anti-Dishevelled 2 antibody staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab228804 was shown to bind specifically to Dishevelled 2. A band was observed at 85-90 kDa in wild-type A549 cell lysates with no signal observed at this size in dvl2 knockout cell line ab277903 (knockout cell lysate ab288317). To generate this image, wild-type and dvl2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-Dishevelled 2 antibody (<a href='/en-us/products/primary-antibodies/dishevelled-2-antibody-ab228804'>ab228804</a>) at 1/5000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
dvl2 knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human DVL2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-dvl2-knockout-a549-cell-line-ab277903'>ab277903</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
THP-1 cell lysate at 20 µg
Predicted band size: 78 kDa
Observed band size: 85 kDa,85-90 kDa
false
- WB
Lab
Western blot - Human DVL2 knockout A549 cell lysate (AB288317)
Lane 1 : Wild-type A549 cell lysate 20 μg
Lane 2 : A549 cell lysate 20 μg
Lane 3 : THP-1 cell lysate 20 μg
Lane 4 : HepG2 cell lysate 20 μg
False colour image of Western blot : Anti-dvl2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to dvl2. A band was observed at 85, 45, 33 kDa in wild-type A549 cell lysates with no signal observed at this size in dvl2 knockout cell line ab277903 (knockout cell lysate None). To generate this image, wild-type and dvl2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Anti-DVL2 Antibody at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
A549 cell lysate at 20 µg
Lane 2:
Western blot - Human DVL2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-dvl2-knockout-a549-cell-line-ab277903'>ab277903</a>)
Lane 3:
THP-1 cell lysate at 20 µg
Lane 4:
HepG2 cell lysate at 20 µg
Secondary
All lanes:
Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution
false
- Sanger seq
Lab
Sanger Sequencing - Human DVL2 knockout A549 cell lysate (AB288317)
88 bp deletion + 1bp mutation (silent) in exon 3
Complementary Products
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com