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AB257414

Human DYNLL1 (PIN) knockout HeLa cell lysate

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DYNLL1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.

View Alternative Names

8 kDa dynein light chain, 8kDLC, Cytoplasmic dynein light polypeptide, DLC8, DNCL1, DNCLC1, DYL1_HUMAN, DYNLL1, Dynein , cytoplasmic, light chain 1, Dynein light chain 1 cytoplasmic, Dynein light chain LC8-type 1, Dynein, cytoplasmic, light polypeptide 1, HDLC1, LC8, LC8a, MGC126137, MGC126138, MGC72986, PIN, Protein inhibitor of neuronal NOS, Protein inhibitor of neuronal nitric oxide synthase

2 Images
Western blot - Human DYNLL1 (PIN) knockout HeLa cell lysate (AB257414)
  • WB

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Western blot - Human DYNLL1 (PIN) knockout HeLa cell lysate (AB257414)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : DYNLL1 knockout HeLa cell lysate (20 μg)

Lane 3 : Mouse testis tissue lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab51603 observed at 10 kDa. Red - loading control ab8245 observed at 36 kDa.

ab51603 Anti-DYNLL1/PIN antibody [EP1660Y] was shown to specifically react with DYNLL1/PIN in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265265 (knockout cell lysate ab257414) was used. Wild-type and DYNLL1/PIN knockout samples were subjected to SDS-PAGE. ab51603 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DYNLL1/PIN antibody [EP1660Y] (<a href='/en-us/products/primary-antibodies/dynll1-pin-antibody-ep1660y-ab51603'>ab51603</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

DYNLL1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human DYNLL1 (PIN) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-dynll1-pin-knockout-hela-cell-line-ab265265'>ab265265</a>)

Lane 3:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 10 kDa

Observed band size: 10 kDa

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Sanger Sequencing - Human DYNLL1 (PIN) knockout HeLa cell lysate (AB257414)
  • Sanger seq

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Sanger Sequencing - Human DYNLL1 (PIN) knockout HeLa cell lysate (AB257414)

Homozygous : 1 bp insertion in exon2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.

Disease

Adenocarcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
DYNLL1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

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Primary Antibodies

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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