Human DYRK1A knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
DYRK1A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon12.
View Alternative Names
DYR1A_HUMAN, DYRK, DYRK 1, DYRKA, Dual specificity YAK 1 related kinase, Dual specificity YAK1-related kinase, Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A, Dual specificity tyrosine phosphorylation regulated kinase, Dual specificity tyrosine phosphorylation regulated kinase 1, Dual specificity tyrosine-phosphorylation-regulated kinase 1A, HP 86, MNB, MNB protein kinase, MNB protein kinase, Serine/threonine-specific, MNB/DYRK protein kinase, MNBH, MRD7, Minibrain (Drosophila) homolog, Minibrain homolog, Minibrain, Drosophila, homolog of, Mnb protein kinase homolog hp86, OTTHUMP00000109090, OTTHUMP00000109091, OTTHUMP00000109094, OTTHUMP00000174799, Protein kinase minibrain homolog, Serine/threonine kinase MNB, hMNB
- WB
Lab
Western blot - Human DYRK1A knockout HEK-293T cell lysate (AB258402)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : HEK-293T cell lysate 20 μg
Lane 3 : HT1080 cell lysate 20 μg
False colour image of Western blot : Anti-DYRK1A antibody staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to DYRK1A. A band was observed at 80/95/90 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in DYRK1A knockout cell line ab267336 (knockout cell lysate ab258402). To generate this image, wild-type and DYRK1A knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
All lanes:
Anti-DYRK1A antibody at 1/500 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human DYRK1A knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-dyrk1a-knockout-hek-293t-cell-line-ab267336'>ab267336</a>)
Lane 3:
HT1080 cell lysate at 20 µg
false
- WB
Lab
Western blot - Human DYRK1A knockout HEK-293T cell lysate (AB258402)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : DYRK1A CRISPR-Cas9 edited HEK-293T cell lysate 20 μg
Lane 3 : HT1080 cell lysate 20 μg
Lane 4 : U-251 MG cell lysate 20 μg
False colour image of Western blot : Anti-DYRK1A antibody [EPR24132-61] staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab259869 was shown to bind specifically to DYRK1A. A band was observed at 80/85/90 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in DYRK1A CRISPR-Cas9 edited cell line ab267336 (CRISPR-Cas9 edited cell lysate ab258402). The band observed in the CRISPR-Cas9 edited lysate lane below 80/85/90 kDa is likely to represent a truncated form of DYRK1A. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and DYRK1A CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-DYRK1A antibody [EPR24132-61] (<a href='/en-us/products/primary-antibodies/dyrk1a-antibody-epr24132-61-ab259869'>ab259869</a>) at 1/2000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
DYRK1A CRISPR-Cas9 edited HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human DYRK1A knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-dyrk1a-knockout-hek-293t-cell-line-ab267336'>ab267336</a>)
Lane 3:
HT1080 cell lysate at 20 µg
Lane 4:
U-251 MG cell lysate at 20 µg
Secondary
All lanes:
Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 80 kDa,85 kDa,90 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human DYRK1A knockout HEK-293T cell lysate (AB258402)
Homozygous : 1 bp insertion in exon12
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB259869
Anti-DYRK1A antibody [EPR24132-61]
20ul selling size|RabMAb|Recombinant
![Western blot - Anti-DYRK1A antibody [EPR24132-61] (AB259869)](https://content.abcam.com/products/images/dyrk1a-antibody-epr24132-61-ab259869--western-blot-img11357.jpg)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com