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AB281354

Human E2F1 knockout HEK-293 cell lysate

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E2F1 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 17 bp deletion, 1 bp insertion; Frameshift = 99%.

View Alternative Names

Dmel\CG6376, Dmel_CG6376, E(Sev-CycE)3A, E(var)3-93E, E2-promoter binding facto, E2F transcription factor 1, E2F1_HUMAN, E2f-PA, E2f-PB, E2f-PC, E2f1 E2F transcription factor 1, Evar(3)164, KIAA4009, OTTHUMP00000030661, PBR3, PRB-binding protein E2F-1, RBAP-1, RBBP-3, RBP 3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1, drosE2F1, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729, mKIAA4009

2 Images
Western blot - Human E2F1 knockout HEK-293 cell lysate (AB281354)
  • WB

Lab

Western blot - Human E2F1 knockout HEK-293 cell lysate (AB281354)

Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 2 : E2F1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate 20 ug

Lane 4 : U-2 OS cell lysate 20 ug

Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lanes 1 - 5 : Merged signal (red and green). Green - ab4070 observed at 55 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

ab4070 was shown to react with E2F1 in wild-type HEK-293 cells in Western blot with loss of signal observed in E2F1 knockout cell line ab269502 (knockout cell lysate ab281354). Wild-type HEK-293 and E2F1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab4070 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-E2F1 antibody [KH95] (<a href='/en-us/products/primary-antibodies/e2f1-antibody-kh95-ab4070'>ab4070</a>) at 1/500 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

E2F1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human E2F1 knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-e2f1-knockout-hek-293-cell-line-ab269502'>ab269502</a>)

Lane 3:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 20 µg

Lane 5:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 47 kDa

Observed band size: 55 kDa

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Next Generation Sequencing - Human E2F1 knockout HEK-293 cell lysate (AB281354)
  • NGS

Supplier Data

Next Generation Sequencing - Human E2F1 knockout HEK-293 cell lysate (AB281354)

Knockout achieved by CRISPR/Cas9; X = 17 bp deletion, 1 bp insertion; Frameshift = 99%

Key facts

Cell type

HEK-293

Species or organism

Human

Tissue

Kidney

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9; X = 17 bp deletion, 1 bp insertion; Frameshift = 99%

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
E2F1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The E2F1 protein also known as E2F transcription factor 1 is an important regulator of the cell cycle. It has a molecular weight of approximately 47 kDa. E2F1 protein is expressed in many tissues with higher expression levels in proliferating cells. It often acts as a transcription factor by binding to specific DNA sequences controlling the transition of cells from G1 to S phase of the cell cycle. This role is vital for the proper progression of the cycle and cellular proliferation.
Biological function summary

E2F1 plays a significant role in cell growth regulation and apoptosis. E2F1 functions within a complex often forming heterodimers with DP proteins. This combination enhances its ability to bind DNA and activate transcription of genes necessary for DNA replication and cell cycle progression. E2F1 also participates in the control of apoptosis by regulating the expression of pro-apoptotic genes balancing cell proliferation and death.

Pathways

E2F1 is deeply embedded in cell cycle regulatory pathways and the p53 signaling pathway. It closely interacts with proteins like RB (Retinoblastoma protein) and p53. RB protein regulates E2F1 activity by controlling its release while p53 helps in mediating the cell's response to DNA damage potentially leading to either cell cycle arrest or apoptosis. These interactions highlight E2F1's role in maintaining cellular homeostasis.

E2F1 is often linked to cancer and neurodegenerative diseases. Overexpression or dysregulation of E2F1 can lead to uncontrolled cell proliferation contributing to oncogenesis. In the context of neurodegenerative diseases E2F1 influences neuronal apoptosis and may be associated with disorders such as Alzheimer's. E2F1 interacts with other proteins such as p53 in cancer where it affects both cell cycle dynamics and apoptosis illustrating its complex involvement in disease progression.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB4070

Anti-E2F1 antibody [KH95]

KO Validated

Western blot - Anti-E2F1 antibody [KH95] (AB4070)

  • 1
  • 1
Proteins & Peptides

AB82207

Recombinant Human E2F1 protein

  • 0
  • 0

Product promise

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For full details, please see our Terms & Conditions

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