Human ECHDC1 knockout HeLa cell lysate
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ECHDC1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 23 bp deletion in exon3 and 71 bp insertion in exon3.
View Alternative Names
ECHD1_HUMAN, Enoyl CoA hydratase domain containing 1, Enoyl Coenzyme A hydratase domain containing 1, Enoyl-CoA hydratase domain-containing protein 1, Uncharacterized hypothalamus protein HCDASE
- Sanger seq
Unknown
Sanger Sequencing - Human ECHDC1 knockout HeLa cell lysate (AB258855)
Allele-1 : 23 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human ECHDC1 knockout HeLa cell lysate (AB258855)
Allele-2 : 71 bp insertion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
EVT-888 (another name for ECHDC1 used in some studies) acts as a component that affects metabolic pathways by mediating reactions associated with energy production. Though it is not actively considered formally as a part of large enzyme complexes ECHDC1 indirectly relates to mitochondrial function and energy regulation. It influences long-chain fatty acid breakdown assisting cells to maintain energy homeostasis.
Pathways
ECHDC1 influences key metabolic pathways like the beta-oxidation pathway of fatty acids and the mitochondrial respiration chain. This protein interacts closely with other proteins such as ACADVL (very long-chain specific acyl-CoA dehydrogenase) and HADHA (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha) playing a central role in energy conversion and mitochondrial efficiency.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB258824
Human COL4A4 (Collagen alpha-4(IV) chain) knockout HeLa cell lysate
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- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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