Human ECI2 (PECI) knockout HEK-293T cell lysate
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ECI2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4.
View Alternative Names
2-trans-enoyl-CoA isomerase, ACBD2, D2-enoyl-CoA isomerase, D3, D3 D2 enoyl CoA isomerase, DBI-related protein 1, DRS-1, Delta(3), Delta(3) delta(2) enoyl CoA isomerase, Diazepam-binding inhibitor-related protein 1, Dodecenoyl-CoA isomerase, ECI2_HUMAN, Enoyl-CoA delta isomerase 2, HCA88, Hepatocellular carcinoma-associated antigen 88, Peroxisomal 3, Peroxisomal 3,2-trans-enoyl-CoA isomerase, Peroxisomal D3 D2 enoyl CoA isomerase, Renal carcinoma antigen NY-REN-1, acyl Coenzyme A binding domain containing 2, delta(2)-enoyl-CoA isomerase, mitochondrial, pECI
- Sanger seq
Unknown
Sanger Sequencing - Human ECI2 (PECI) knockout HEK-293T cell lysate (AB263179)
Homozygous : 1 bp insertion in exon 4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PECI contributes to the metabolism of fatty acids by ensuring effective breakdown of unsaturated fatty acids in peroxisomes. The enzyme participates as a solitary player and not part of a larger protein complex. By converting cis-double bonds to the trans configuration PECI aids in stabilizing the substrate for further catabolic processing. This facilitated breakdown is critical for energy production especially during fasting when fatty acids become a significant energy source.
Pathways
PECI's mechanical function integrates into the larger pathway of fatty acid metabolism especially the peroxisomal beta-oxidation pathway. This pathway teams with enzymes such as acyl-CoA oxidase and multifunctional enzyme protein to facilitate complete fatty acid degradation. PECI's transformation of unsaturated fatty acids ensures these substrates enter the pathway's main cycle aiding in continued lipid metabolism and maintaining cellular energy balance.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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