Human EEF1E1 (AIMP3/p18) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human EEF1E1 (AIMP3/p18) knockout HEK-293T cell lysate (AB258404)
Homozygous : Insertion of the selection cassette in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The role of AIMP3 extends beyond its contribution to protein synthesis. This protein participates in the macromolecular ARS complex which is essential for accurate and efficient translation of the genetic code into functional proteins. AIMP3 acts as a scaffold stabilizing the assembly and function of the ARS complex. Its involvement is critical for maintaining the fidelity of protein synthesis as errors in this process can lead to cellular dysfunction.
Pathways
AIMP3 engages in several key biological processes. It functions within the DNA damage response pathway where it interacts with proteins such as ATM and ATR to facilitate DNA repair mechanisms. Additionally AIMP3 is involved in the p53 signaling pathway where it contributes to the regulation of cell cycle and apoptosis. This interaction highlights the protein's role in maintaining genomic stability and preventing tumorigenesis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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