Human EIF1 knockout HEK-293T cell lysate
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EIF1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 44 bp deletion in exon 1.
View Alternative Names
EIF1_HUMAN, Eukaryotic translation initiation factor 1, ISO1, Protein translation factor SUI1 homolog, SUI1, Sui1iso1, eIF-1A
- Sanger seq
Unknown
Sanger Sequencing - Human EIF1 knockout HEK-293T cell lysate (AB263181)
Homozygous : 44 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Eukaryotic translation initiation factor 1 facilitates the formation of the translation pre-initiation complex. It is an integral component of the eIF1A- and eIF2-associated assembly that is important for the proper translation initiation. This factor acts to stabilize the pre-initiation complex by preventing premature joining with the 60S ribosomal subunit ensuring accurate translation initiation sites are selected across a range of mRNAs.
Pathways
EIF1 is primarily involved in the mRNA translation initiation pathway. This pathway is important for regulating protein synthesis and gene expression. eIF1 interacts closely with other initiation factors like eIF2 which carries the initiator methionyl-tRNA and eIF3 which binds to the 40S ribosomal subunit to form the functional ribosome assembly. Additionally eIF1’s role in translation initiation is also linked to cellular stress responses influencing how cells adapt to changing environmental conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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