Human EIF2AK2 (PKR) knockout A549 cell lysate
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EIF2AK2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon5 and 1 bp deletion in exon5 and 1 bp insertion in exon5.
View Alternative Names
Double stranded RNA activated protein kinase;, E2AK2_HUMAN, EIF2AK1, EIF2AK2, Eukaryotic translation initiation factor 2-alpha kinase 2, HGNC:9437, Interferon inducible elF2 alpha kinase, Interferon-induced, double-stranded RNA-activated protein kinase, Interferon-inducible RNA-dependent protein kinase, MGC126524, P1/eIF-2A protein kinase, PPP1R83, PRKR, Protein kinase RNA-activated, Protein kinase interferon inducible double stranded RNA dependent, Protein phosphatase 1 regulatory subunit 83, Serine/threonine protein kinase TIK, Tyrosine protein kinase EIF2AK2, eIF-2A protein kinase 2, p68 kinase
- WB
Lab
Western blot - Human EIF2AK2 (PKR) knockout A549 cell lysate (AB256901)
Lane 1 : Wild-type A549 cell lysate (20 ug)
Lane 2 : EIF2AK2 knockout A549 cell lysate (20 ug)
Lane 3 : K-562 cell lysate (20 ug)
ab32052 was shown to specifically react with PKR in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267000 (knockout cell lysate ab256901) was used. Wild-type and PKR knockout samples were subjected to SDS-PAGE. ab32052 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PKR antibody [YE350] (<a href='/en-us/products/primary-antibodies/pkr-antibody-ye350-ab32052'>ab32052</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
EIF2AK2 knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human EIF2AK2 (PKR) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-eif2ak2-pkr-knockout-a549-cell-line-ab267000'>ab267000</a>)
Lane 3:
K-562 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 62 kDa
Observed band size: 70 kDa
false
- WB
Lab
Western blot - Human EIF2AK2 (PKR) knockout A549 cell lysate (AB256901)
Lane 1 : Wild-type A549 cell lysate (20 ug)
Lane 2 : EIF2AK2 knockout A549 cell lysate (20 ug)
Lane 3 : K-562 cell lysate (20 ug)
ab184257 was shown to specifically react with PKR in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267000 (knockout cell lysate ab256901) was used. Wild-type and PKR knockout samples were subjected to SDS-PAGE. ab184257 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PKR antibody [EPR19374] (<a href='/en-us/products/primary-antibodies/pkr-antibody-epr19374-ab184257'>ab184257</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
EIF2AK2 knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human EIF2AK2 (PKR) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-eif2ak2-pkr-knockout-a549-cell-line-ab267000'>ab267000</a>)
Lane 3:
K-562 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 62 kDa,86 kDa
Observed band size: 70 kDa,86 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human EIF2AK2 (PKR) knockout A549 cell lysate (AB256901)
Allele-1 : 10 bp deletion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human EIF2AK2 (PKR) knockout A549 cell lysate (AB256901)
Allele-2 : 1 bp deletion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human EIF2AK2 (PKR) knockout A549 cell lysate (AB256901)
Allele-3 : 1 bp insertion in exon5
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Primary Antibodies
AB184257
RabMAb|Recombinant|KO Validated|20ul selling size
![Immunocytochemistry/ Immunofluorescence - Anti-PKR antibody [EPR19374] (AB184257)](https://content.abcam.com/products/images/pkr-antibody-epr19374-ab184257--immunocytochemistry-immunofluorescence-img424954.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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