Human EIF2D knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human EIF2D knockout HEK-293T cell lysate (AB258856)
Homozygous : 1 bp insertion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
EIF2D influences protein synthesis through its role in translation initiation and ribosome recycling. It may operate as a part of a complex potentially interacting with other factors involved in translation setup. This role is fundamental to maintaining cellular homeostasis and responding to cellular stress by adjusting protein production rates. EIF2D's interaction with the ribosome and its factors points to its active involvement in the complex regulation of cellular protein levels.
Pathways
EIF2D takes part in the translation initiation pathway which involves other proteins such as eIF1 and eIF2. These factors work together to ensure proper recognition of start codons on mRNA strands. EIF2D also fits into the mRNA surveillance pathway which is responsible for ensuring the fidelity of mRNA translation. The accuracy of this process is critical for proper cellular function and response to stress or damage signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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