Human EIF3K knockout HEK-293T cell lysate
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(0 Publication)
- WB
Lab
Western blot - Human EIF3K knockout HEK-293T cell lysate (AB258857)
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 2 : EIF3K knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 3 : THP1 (Human monocytic leukemia cell line) whole cell lysate (20 ug)
ab50736 was shown to specifically react with eIF3K in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266841 (knockout cell lysate ab258857) was used. Wild-type and eIF3K knockout samples were subjected to SDS-PAGE. ab50736 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4oC at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-eIF3K antibody [2313C2a] (<a href='/en-us/products/primary-antibodies/eif3k-antibody-2313c2a-ab50736'>ab50736</a>) at 1/500 dilution
Lane 1:
Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2:
EIF3K knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2:
Western blot - Human EIF3K knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-eif3k-knockout-hek-293t-cell-line-ab266841'>ab266841</a>)
Lane 3:
THP1 (Human monocytic leukemia cell line) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human EIF3K knockout HEK-293T cell lysate (AB258857)
Homozygous : 64 bp deletion in exon 1
Complementary Products
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
EIF3K is involved in the regulation of the eukaryotic translation initiation factor 3 complex which is essential for initiating translation by serving as a scaffold for other eIF3 subunits. This complex stabilizes the formation of the pre-initiation complex necessary for ribosome binding to mRNA. Through its involvement in protein synthesis eIF3K influences cell growth proliferation and response to various cellular signals. It is involved in processes such as cell cycle control and apoptotic regulation as it aids the translation of specific mRNAs coding for key regulatory proteins.
Pathways
EIF3K participates in mTOR and Wnt signaling pathways. These pathways are critical in controlling cell growth proliferation and differentiation. Through mTOR signaling eIF3K interacts with proteins like ribosomal protein S6 kinase beta-1 (S6K1) influencing cell metabolism and growth. In the Wnt pathway eIF3K contributes to the translation of proteins that regulate cell fate and stem cell renewal. Interactions with these pathways illustrate the importance of eIF3K in maintaining cellular homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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