Human EIF4G2 (P97/DAP5) knockout HEK-293T cell lysate
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EIF4G2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 128 bp insertion in exon3.
View Alternative Names
DAP-5, Death-associated protein 5, Eukaryotic translation initiation factor 4 gamma 2, IF4G2_HUMAN, Novel APOBEC-1 target 1, P97, Translation repressor NAT1, eIF-4-gamma 2, eIF-4G 2
- Sanger seq
Unknown
Sanger Sequencing - Human EIF4G2 (P97/DAP5) knockout HEK-293T cell lysate (AB263183)
Homozygous : 128 bp insertion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P97/DAP5 impacts translation initiation by interacting with components of the translation machinery. It is part of a multiprotein complex where it binds to other translation initiation factors. Unlike eIF4G1 P97/DAP5 operates in conditions where cap-dependent translation is downregulated such as during cellular stress. This activity highlights its role in selectively activating the translation of mRNAs that are important for cell survival and adaptation during times of cellular distress.
Pathways
P97/DAP5 plays critical roles in the cellular stress response and apoptosis pathways. In the stress response pathway it is instrumental in shifting translation from a cap-dependent to cap-independent mechanism often in cooperation with proteins like eIF4B. In the apoptosis pathway it modulates the translation of apoptotic factors. These functions ensure cells maintain protein synthesis under adverse conditions maintaining cellular viability and regulating programmed cell death.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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