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AB256904

Human ELK1 knockout HeLa cell lysate

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ELK1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 222 bp insertion in exon 2.

View Alternative Names

ELK1 member of ETS oncogene family, ELK1 protein, ELK1, ETS transcription factor, ELK1_HUMAN, ELK2 member of ETS oncogene family, ETS domain protein Elk1, ETS domain-containing protein Elk-1, ETS like gene 1, Member of ETS oncogene family, Oncogene Elk1, Tyrosine kinase (ELK1) oncogene

3 Images
Western blot - Human ELK1 knockout HeLa cell lysate (AB256904)
  • WB

Lab

Western blot - Human ELK1 knockout HeLa cell lysate (AB256904)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : ELK1 knockout HeLa cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab188316 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

ab188316 Anti-ELK1 antibody [EPR1913(2)] was shown to specifically react with ELK1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261764 (knockout cell lysate ab256904) was used. Wild-type and ELK1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab188316 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ELK1 antibody [EPR1913(2)] (<a href='/en-us/products/primary-antibodies/elk1-antibody-epr19132-ab188316'>ab188316</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ELK1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ELK1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-elk1-knockout-hela-cell-line-ab261764'>ab261764</a>)

Predicted band size: 45 kDa

Observed band size: 55 kDa

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Western blot - Human ELK1 knockout HeLa cell lysate (AB256904)
  • WB

Lab

Western blot - Human ELK1 knockout HeLa cell lysate (AB256904)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : ELK1 knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab32106 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32106 Anti-ELK1 antibody [E277] - ChIP Grade was shown to specifically react with ELK1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261764 (knockout cell lysate ab256904) was used. Wild-type and ELK1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32106 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ELK1 antibody [E277] (<a href='/en-us/products/primary-antibodies/elk1-antibody-e277-ab32106'>ab32106</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ELK1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ELK1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-elk1-knockout-hela-cell-line-ab261764'>ab261764</a>)

Predicted band size: 45 kDa

Observed band size: 55 kDa

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Sanger Sequencing - Human ELK1 knockout HeLa cell lysate (AB256904)
  • Sanger seq

Unknown

Sanger Sequencing - Human ELK1 knockout HeLa cell lysate (AB256904)

Homozygous : 222 bp insertion in exon 2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 222 bp insertion in exon 2.

Disease

Adenocarcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
ELK1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELK1 also known as ELK-1 or ETS-domain protein ELK1 is a transcription factor involved in regulating gene expression. It is a member of the ETS family of transcription factors and has a molecular mass of approximately 54 kDa. ELK1 is mainly expressed in the nucleus of cells and plays a significant role in various tissues by interacting with other proteins. It has sequence-specific DNA-binding ability which allows it to influence the transcription of target genes.
Biological function summary

ELK1 functions in the regulation of gene expression related to cellular proliferation and differentiation. It often forms a ternary complex with serum response factor (SRF) on serum response elements (SREs) to modulate the transcriptional responses of genes important for cell growth. The activation of ELK1 is phosphorylation-dependent primarily by mitogen-activated protein kinases (MAPKs) which further facilitates its role as a transcription regulator. This phosphorylation increases the transcriptional activity enabling ELK1 to actively participate in cellular responses to external signals.

Pathways

ELK1 participates prominently in the MAPK/ERK signaling pathway and the JNK signaling pathway. Through these pathways ELK1 gets activated influencing the transcription of immediate early genes responsible for cell cycle progression and apoptosis. ELK1 interacts with other proteins in these pathways such as MEK and ERK leading to a coordinated response to growth signals. These interactions underline its importance in signal transduction processes and cellular response mechanisms.

ELK1 has associations with certain cancers and neurodegenerative disorders. In particular its dysregulation has been noted in the context of breast cancer where ELK1 is implicated in abnormal cell proliferation. Similarly in neurodegenerative diseases like Alzheimer's ELK1's altered activity levels can affect neuron function and survival. Other proteins such as amyloid precursor protein in Alzheimer's or estrogen receptor in breast cancer connect with ELK1 influencing disease progression through complex signaling networks.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB32106

Anti-ELK1 antibody [E277]

RabMAb|Recombinant|KO Validated|Advanced Validation

ChIC/CUT&RUN sequencing - Anti-ELK1 antibody [E277] (AB32106)

  • 0
  • 0
Proteins & Peptides

AB70380

Recombinant Human ELK1 protein

SDS-PAGE - Recombinant Human ELK1 protein (AB70380)

  • 0
  • 0

Product promise

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