AB257939

Human EMC10 (C19orf63) knockout HeLa cell lysate

Name: Human EMC10 (C19orf63) knockout HeLa cell lysate (ab257939) | Abcam
Brand: Abcam Limited
SKU: AB257939
Price: 670 USD
Availability: InStock

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EMC10 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 55 bp deletion in exon1 and 73 bp deletion in exon1.

View Alternative Names

Chromosome 19 open reading frame 63, EMC10_HUMAN, ER membrane protein complex subunit 10, HSM1, HSS1, Hematopoietic signal peptide containing membrane domain containing 1, Hematopoietic signal peptide containing secreted 1, Hematopoietic signal peptide-containing membrane domain-containing protein 1, INM02, UPF0510 protein INM02

4 Images

Lab

Western blot - Human EMC10 (C19orf63) knockout HeLa cell lysate (AB257939)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : EMC10 knockout HeLa cell lysate (20 μg)

Lane 3 : A549 cell lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab180148 observed at 27 kDa. Red - loading control ab7291 observed at 50 kDa.

ab180148 Anti-C19orf63 antibody [EPR13223-65] was shown to specifically react with C19orf63 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265783 (knockout cell lysate ab257939) was used. Wild-type and C19orf63 knockout samples were subjected to SDS-PAGE. ab180148 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-C19orf63 antibody [EPR13223-65] (<a href='/en-us/products/primary-antibodies/c19orf63-antibody-epr13223-65-ab180148'>ab180148</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

EMC10 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human EMC10 (C19orf63) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-emc10-c19orf63-knockout-hela-cell-line-ab265783'>ab265783</a>)

Lane 3:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

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Lab

Western blot - Human EMC10 (C19orf63) knockout HeLa cell lysate (AB257939)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : EMC10 knockout HeLa cell lysate (20 μg)

Lane 3 : A549 cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab181209 observed at 27 kDa. Red - loading control ab7291 observed at 50 kDa.

ab181209 Anti-C19orf63 antibody [EPR13223] was shown to specifically react with C19orf63 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265783 (knockout cell lysate ab257939) was used. Wild-type and C19orf63 knockout samples were subjected to SDS-PAGE. ab181209 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-C19orf63 antibody [EPR13223] (<a href='/en-us/products/primary-antibodies/c19orf63-antibody-epr13223-ab181209'>ab181209</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

EMC10 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human EMC10 (C19orf63) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-emc10-c19orf63-knockout-hela-cell-line-ab265783'>ab265783</a>)

Lane 3:

A549 cell lysate at 20 µg

Secondary

All lanes:

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Sanger Sequencing - Human EMC10 (C19orf63) knockout HeLa cell lysate (AB257939)

Sanger seq

Unknown

Sanger Sequencing - Human EMC10 (C19orf63) knockout HeLa cell lysate (AB257939)

Allele-1 : 73 bp deletion in exon1

Sanger seq

Unknown

Sanger Sequencing - Human EMC10 (C19orf63) knockout HeLa cell lysate (AB257939)

Allele-2 : 55 bp deletion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 55 bp deletion in exon1 and 73 bp deletion in exon1.

Disease

Adenocarcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257939-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human EMC10 knockout HeLa cell lysate", "number":"AB257939-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB257939-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Gene name

EMC10

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing, Western blot

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The C19orf63 protein also known as MRG domain-binding protein (MRGBP) works mechanically as a component of the histone acetyltransferase complex. It weighs approximately 19 kDa and is expressed in various human tissues with notable expression in HeLa cells. This protein is integral to chromatin modifications influencing transcription regulation through histone acetylation.

Biological function summary

C19orf63 interacts within the histone acetyltransferase complex playing a role in the modification of chromatin structure. It participates in transcriptional regulation by modulating the acetylation state of histones thereby impacting gene expression. This protein does not work alone and requires interaction with other proteins to execute its function efficiently.

Pathways

C19orf63 operates in pathways related to chromatin modification and transcription control. The protein influences the regulation of transcriptional signaling pathways such as the acetylation-dependent signaling pathway. C19orf63 associates with proteins like PCAF a known histone acetyltransferase working together to maintain gene accessibility and transcription fidelity.

C19orf63 has connections with certain cancers through its role in chromatin structure and function alteration. Abnormal expression of this protein might contribute to tumorigenesis by altering gene expression profiles. Through the histone modification pathway it connects with proteins like BRCA1 which is involved in DNA repair and is associated with breast cancer.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human EMC10 (C19orf63) knockout HeLa cell lysate

Western blot - Human EMC10 (C19orf63) knockout HeLa cell lysate (AB257939)

Western blot - Human EMC10 (C19orf63) knockout HeLa cell lysate (AB257939)

Sanger Sequencing - Human EMC10 (C19orf63) knockout HeLa cell lysate (AB257939)

Sanger Sequencing - Human EMC10 (C19orf63) knockout HeLa cell lysate (AB257939)

Key facts

Cell type

Species or organism

Tissue

Knockout validation

Mutation description

Disease

Reactivity data

Product details

What's included?

Properties and storage information

Gene name

Gene editing type

Gene editing method

Knockout validation

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Quality control

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

Product protocols

Complementary Products

Primary Antibodies

Proteins & Peptides

Proteins & Peptides

Cell Lines & Lysates

Product promise