Human EMC6 (Transmembrane protein 93) knockout HeLa cell lysate
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EMC6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 23 bp deletion in exon 2 and 94 bp deletion in exon 2.
View Alternative Names
0610009E20Rik, 0610025L18Rik, MGC2963, OTTMUSP00000006414, OTTMUSP00000006415, RGD1309231, RP23-263M10.8, TMEM93, Transmembrane protein 93
- Sanger seq
Unknown
Sanger Sequencing - Human EMC6 (Transmembrane protein 93) knockout HeLa cell lysate (AB258858)
Allele-1 : 94 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human EMC6 (Transmembrane protein 93) knockout HeLa cell lysate (AB258858)
Allele-2 : 23 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human EMC6 (Transmembrane protein 93) knockout HeLa cell lysate (AB258858)
Allele-3 : 1 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein interacts with other components in the endoplasmic reticulum and is essential for proper folding and insertion of proteins into the ER membrane. EMC6 functions as part of a larger scaffolding complex that supports ER-associated degradation (ERAD) which is necessary for maintaining protein homeostasis. This complex plays an important role in quality control by removing misfolded proteins and EMC6 specifically contributes to the stabilization and function of this complex.
Pathways
EMC6 has important roles in the ER stress response and autophagy pathways. The protein interacts with autophagy-related proteins including Beclin 1 facilitating the cellular recycling process. Through the ER stress response EMC6 participates in maintaining cellular health by assisting in protein refolding and degradation pathways linking its function to the action of other ER stress-related proteins like IRE1 and PERK.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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