Human EMC8 (COX4NB) knockout HeLa cell lysate
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EMC8 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
C16orf2, C16orf4, COX4 neighbor, COX4, neighbor of, COX4AL, CX4NB_HUMAN, ER membrane protein complex subunit 8, FAM158B, NOC4, Neighbor of COX4, Protein FAM158B, family with sequence similarity 158, member B
- Sanger seq
Unknown
Sanger Sequencing - Human EMC8 (COX4NB) knockout HeLa cell lysate (AB258859)
Allele-2 : Insertion of the selection cassette in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human EMC8 (COX4NB) knockout HeLa cell lysate (AB258859)
Allele-1 : 1 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
COX4NB participates in mitochondrial function. The protein integrates into the cytochrome C oxidase complex which is an important component of the electron transport chain in mitochondria. Through this involvement COX4NB contributes to cellular respiration and ATP production which maintain energy homeostasis in cells.
Pathways
COX4NB is involved in key metabolic pathways. It is part of the oxidative phosphorylation pathway which is essential for the generation of ATP in aerobic organisms. In this process COX4NB works alongside proteins like COX4I1 and other subunits of the cytochrome C oxidase complex to facilitate efficient electron transfer and energy production.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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